SPRR1A基因真核荧光表达载体的构建  

Construction for fluorescent eukaryotic cell expression vector of small proline-rich repeat protein 1A gene

作　　者：张鼎[1] 孙西美[2] 邵正仁[3] 陈传好[3] 

机构地区：[1]蚌埠医学院生物科学系实验中心,安徽蚌埠233030 [2]蚌埠医学院图书馆,安徽蚌埠233030 [3]蚌埠医学院人体解剖学教研室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2009年第2期97-99,共3页Journal of Bengbu Medical College

基　　金：安徽省高校自然科学研究资助项目(KJ2007B205);安徽省教育厅优秀青年科研项目(2005jq1121);蚌埠医学院科研课题(BY0303)

摘　　要：目的:构建可在真核细胞中表达SPRR1A(small proline-rich repeat protein1A)的荧光表达载体。方法:应用PCR技术扩增出编码SPRR1A的全长基因,将SPRR1A DNA克隆到pEGFP-N1质粒,通过抗性基因筛选阳性克隆,经酶切和测序鉴定。结果:酶切和DNA序列鉴定均证实插入片段与GenBank提供的序列(L05187)一致。结论:成功构建了SPRR1A真核荧光表达载体。Objective：To construct fluorescent eukaryotic expression vector of small proline-rich repeat protein 1 A （SPRR1 A） gene. Methods：The coding sequence of SPRR1A was amplified by polymerase chain reaction, the SPRR1 A gene was cloned into plasmid pEGFP-N1, and the recombinant vector was selected and identified by restriction enzyme analysis and nucleotide sequence determination. Results： Correct construction of pEGFP-N1-SPRR1A was identified by methods of restriction enzyme analysis and nucleotide sequence determination. Conclusions： Eukaryotic expression plasmid pEGFP-NI-SPRR1A has been successfully

关 键 词：神经元纤维 基因表达 调节 SPRR1A 真核荧光表达载体 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学] Q343.1[医药卫生—基础医学]