辛伐他汀影响内皮祖细胞数量、黏附及凋亡的研究  

Study on the effects of simvastatin on the number,adhesion and apoptosis of endothelial progenitor cells

作　　者：周亚林[1] 杨乃全[1] 方翔[1] 张雅莉[1] 朱源生[1] 梅介平[1] 

机构地区：[1]徐州医学院附属淮安第二人民医院心脏内科,江苏淮安223001

出　　处：《徐州医学院学报》2009年第2期86-88,共3页Acta Academiae Medicinae Xuzhou

摘　　要：目的细胞水平观察不同浓度辛伐他汀对内皮祖细胞(endothelial progenitor cells,EPCs)功能的影响。方法采用密度梯度离心法从人外周血获取单个核细胞,将其接种在人纤维连接蛋白包被培养板上,培养7天后,收集贴壁细胞,通过激光共聚焦显微镜鉴定,FITC-UEA-I和DiI-acLDL双染色阳性细胞为正在分化EPCs。以不同浓度辛伐他汀(分别为0.0001、0.001、0.01、0.1、1.0μmol/L)和EPCs培养。分别采用黏附能力测定实验、碘化丙啶(PI)标记观察辛伐他汀对EPCs黏附与凋亡的影响。结果辛伐他汀显著提高了外周血EPCs的数量、黏附能力,并能抑制其凋亡。辛伐他汀浓度在0.01μmol/L时对EPCs的数量、黏附功能和抗凋亡能力影响达到最大。随着药物浓度的继续增大,EPCs的上述功能反呈下降趋势,但0.1μmol/L组仍高于对照组。结论辛伐他汀能增加EPCs数量、黏附能力并能抑制其凋亡。Objective To investigate the effects of simvastatin on EPC number, adhesion and apoptosis. Methods Total mononuclear cells （ MNCs ） isolated from human peripheral blood by Ficoll density gradient eentrifugation were plated on fibronectin - coated culture dishes. After 7 days of culture, attached cells were obtained and assessed with a laser scanning eonfocal microscope, and differentiating EPCs were characterized as adherent positive cells by double staining of DiLDL - uptake and lectin binding. EPCs were treated with simvastatin of different concentrations （0. 0001, 0. 001, 0.01 , 0.1 and 1.0 umol/L）. EPC adhesion assay was performed by adherent cell counting on fibronectin - coated culture dishes. EPC apoptosis was evaluated by flow eytometry. Results Simvastatin promoted EPC number and adhesive capacity and prevented apoptosis . Simvastatin at 0.01 umol/L had the maximum effects on EPCs number, adhesion and anti - apoptosis. While the concentration of simvastatin further increased more than 0. 01 umol/L, the above improvement effects showed a tendency to decline, but the effects at 0. 1 umoL/L concentration were still greater than those in control group. Conclusion The study establishes that simvastatin could improve EPCs number and adhesion and prevent apoptosis.

关 键 词：他汀类药物 内皮祖细胞 黏附 凋亡 

分 类 号：R927.6[医药卫生—药学]