癫癎发作过程中细胞凋亡与一氧化氮合酶、核因子-B的关系  

Relationship of nitric oxide synthase,NF-κB and cell apoptosis in epilepsy

作　　者：刘月影 邓星强[1] 李瑞 袁宝强 

机构地区：[1]徐州市儿童医院神经内科,江苏徐州221002 [2]无锡市第四人民医院儿科 [3]徐州医学院附属医院儿科,江苏徐州221002

出　　处：《徐州医学院学报》2009年第2期111-113,共3页Acta Academiae Medicinae Xuzhou

基　　金：徐州市科技计划项目(2006-35)

摘　　要：目的探讨癫癎发作过程中细胞凋亡与一氧化氮合酶(NOS)、核因子-B(NF-B)的关系。方法采用戊四氮(PTZ)致癎大鼠模型,检测癫癎发作后神经细胞凋亡、NOS和NF-B的变化。结果细胞凋亡率在癫癎发作后6 h开始升高,至24 h达到高峰,48 h下降,与对照组比较差异有显著性(P<0.05)。NOS在癫癎发作后1 h、24 h有2个分泌高峰,与对照组比较差异有显著性(P<0.05);对照组大鼠海马没有NF-B核转位细胞,而致癎后NF-B核转位细胞显著上调,24 h达高峰(P<0.01)。结论PTZ致癎大鼠模型中NOS早期可能参与癫癎发生,后期可能通过诱导NF-B产生而参与了神经细胞的凋亡。Objective To investigate the relationship of nitric oxide synthase （ NOS）, nuclear factor - KB （ NF - KB） and cell apoptosis in epilepsy. Methods Animal model of epilepsy was established by pentylenetetrazol （PTZ） induction in rats. Following epilepsy seizure, the variations of apoptosis levels of the nerve cells at the different times, NOS and the activity of NF - KB in the rat hippocampus were determined by flow cytometry （ FCM ） , chromatometry and immunohistochemistry, respectively. Results The apoptosis rate increased 6 h after seizure, reached its peak at 24 h and decreased at 48 h. The statistical differences were considered significant compared with the control group （ P 〈 0.05 ）. The levels of NOS had two respective secretion peaks 1 b and the 24 h after epilepsy, and had the significant differences compared with the control group （ P 〈 0.05 ）. In the control group, there were no cells with nuclear translocation of NF - KB in the rat hippocampus, while the expression of cells with nuclear translocation of NF - KB in the PTZ group was remarkably upregulated and reached its peak 24 h after seizure （ P 〈 0.01 ）. Conclusion In the PTZ group, NOS may participate in the seizure generation in the early stage, while in the latter stage it may be involved in cell apoptosis by inducing the activation of NF - KB.

关 键 词：癫癎 细胞凋亡 一氧化氮合酶 NF-B 

分 类 号：R729[医药卫生—儿科]