鲫鱼斯坦尼小体组织匀浆对其血清离子钙浓度降低作用的研究  

Observation on Reducing Effect of Stannius Corpuscles Homogenate of Carassius auratus on Its Serum Ionic Calcium Concentration

作　　者：仇存网[1] 袁挺[1] 沈敏[1] 从默[2] 

机构地区：[1]盐城师范学院江苏省滩涂生物资源与环境保护重点建设实验室,江苏盐城224051 [2]南京大学生命科学院,江苏南京210093

出　　处：《安徽农业科学》2009年第2期620-621,649,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省高校自然科学基础研究项目(08KJD210004);江苏省滩涂生物资源与环境保护重点建设实验室资助项目(JLCBE07027)资助

摘　　要：[目的]检测鲫鱼斯坦尼小体组织匀浆对其血清离子钙的降低作用。[方法]将选购自农贸市场体重约200 g的健康鲫鱼在加入EDTA.2Na.2H2O自来水的低钙环境中饲养8周,之后用斯坦尼小体组织匀浆按每100 g体重1.0 m l的剂量进行体腔注射,以0.6%NaC l溶液作对照注射,注射后于不同时间断尾取血,制备血清,测量血清钙离子的浓度。[结果]鲫鱼饲养在低钙水中8周,其血清离子钙浓度没有统计上的变化,却使其对CS组织匀浆产生应答性的低血钙反应。30 mg/100 g.b.w的早春鲫鱼斯坦尼小体组织匀浆,对其正常生理水平的血清离子钙有明显的降低效应,该效应至少可持续到注入匀浆后的72 h。最佳效应时间为注入后的48 h,此时的Ca2+浓度尚达不到对照组的80%,低血钙效应大约在98 h后消失。[结论]研究推测,鱼类在排卵中或排卵后可能处于低血钙状态。[ Objective ] The study aimed to test the reducing effect of Stannius corpuscles homogenate of crucian （ Curassius auratus） on its serum ionic calcium. [ Method ] The healthy erucian weighted about 200 g, which was purchased at farm products market, was fed in low-calcium running water for 8 weeks, with EDTA · 2Na · 2H2O adding into the water, and then treated with intravitreous injection by Stannius corpuscles homogenate as per the dosage of I. 0 ml/100 g body weight, and 0.6% NaCl solution was used as control injection, then blood was taken out from the cutting tail after injection in different time, serum was prepared and calcium ionic conch, in serum was measured. [ Result] When crucian was raised in low-calcium water for 8 weeks, there was no statistic change on its calcium ionic concn, in serum, but made fish produce responsive hypocalcemia reaction to CS bomogenate. The Stannius corpuscles homogenates of early spring crucian at 30 mg/100 g. b. w had significant reducing effect on its calcium ionic eoncn, in serum at normal physiological level, the effect could last 72 h at least after injecting homogenate. The optimal effect time was 48 h after homogenate injection, the Ca^2＋ concn, at this time could not attain 80% of control group, the hypocalcemic effect disappeared about 98 h later. [ Conclusion] The research conjectured that fish might in hypocalcemic state during ovulationor or after ovulationor.

关 键 词：鲫鱼 斯坦尼小体 血清离子钙浓度 低血钙效应 时效 

分 类 号：S965.117[农业科学—水产养殖]