纽荷尔脐橙及其芽变系的RAPD分析  

RAPD Analysis on Newhall Navel Orange and Its Variant Line

作　　者：范建新[1] 桂阳[1,2] 李金强[1] 

机构地区：[1]贵州省果树科学研究所,贵州罗甸550100 [2]贵州省农业生物技术重点实验室,贵州贵阳550006

出　　处：《贵州农业科学》2009年第2期121-122,共2页Guizhou Agricultural Sciences

基　　金：贵州省农业动植物育种专项"长圆形纽荷尔脐橙变异株分子标记鉴定及栽培利用研究"[黔农育专字(2007)023]

摘　　要：采用RAPD分析,从60个引物中筛选出能稳定扩增的12个引物,对2个纽荷尔脐橙品种(系)进行了扩增,共扩增出91条带,1条具有多态性,多态性比例占1.10%。利用NTSYSpc2.1软件进行统计分析,得到品种(系)间的遗传距离极小,为0.0055。DNA of Newhall navel orange and its variant line was amplified by 12 primers with stable amplification selected from 60 primers. The results showed that 91 bands were amplified and only one band was of polymorphism. The genetic distance between Newhall navel orange and its variant line was 0. 0055 by using the cluster analysis.

关 键 词：RAPD分析 纽荷尔脐橙 遗传距离 

分 类 号：S666.4[农业科学—果树学]