血小板活化对单采血小板制剂中VEGF、TGF-β_1和PDGF含量的影响  

Effect of platelet activation on the levels of VEGR,TGF-β_1 and PDGF in apheresis platelets

作　　者：李静[1] 黎儒青[1] 王昕[1] 赵树铭[1] 

机构地区：[1]第三军医大学西南医院输血科,重庆400038

出　　处：《中国输血杂志》2009年第1期23-25,共3页Chinese Journal of Blood Transfusion

基　　金：第三军医大学西南医院临床科研创新基金(2008)

摘　　要：目的了解血管内皮细胞生长因子(VEGF)、转化生长因子-β1(TGF-β1)、血小板源性生长因子(PDGF)在单采血小板制剂中血小板活化过程中的含量变化。方法选取10名单采(双份)血小板献血者,分别留取血浆(3ml/人,作为对照组)、新鲜单采血小板(采集当日的血小板,5ml/人,作为实验1组)和常规保存单采血小板(22℃振荡箱中保存5d的血小板,5ml/人,作为实验2组);用血细胞分析仪分别测定各组血小板计数(Plt),用酶联免疫吸附试验(ELISA)测定这3组样本中VEGF、TGF-β1、PDGF的含量,用流式细胞术测定2个实验组的CD62p表达,并比较分析检测结果。结果新鲜单采血小板中的VEGF、TGF-β1、PDGF含量[分别为(410.95±95.07)pg/ml、(91.15±19.50)ng/ml和(12.60±2.06)ng/ml]明显高于新鲜血浆中相对应的各生长因子水平[分别为(149.09±28.11)pg/ml、(37.38±10.73)和(3.28±0.79)ng/ml],差异具统计学意义(P<0.01);常规保存血小板中VEGF、TGF-β1和PDGF含量[分别为(495.16±63.49)pg/ml、(110.33±19.06)和(16.96±2.71)ng/ml]又高于新鲜单采血小板中相应的各生长因子水平,差异也有统计学意义(P<0.05)。Plt与VEGF、TGF-β1、PDGF呈一定程度的正相关,Plt及其活化均影响生长因子含量。结论单采血小板中VEGF、TGF-β1和PDGF水平呈高表达,血小板活化可促进这些因子的产生和释放。Objective To investigate the changes of vascular endothelial growth factor （ VEGF）, transforming growth factor-β1 （ TGF-β1 ） and platelet derived growth faetor（ PDGF） in plasma and apheresis platelets with or without activation by shaking. Methods A total of 10 units of double therapeutic dose platelets were collected and each unit was divided into 3 groups to take samples of plasma （3ml/person, as eontrol group）, fresh apheresis platelets （5ml/person, as experimental groupl ） and activated apheresis platelets （platelets stored by shaking at 22 ℃ for 5 days, 5ml/person, as experimental group2 ）. The levels of VEGF, TGF-β1 and PDGF in the samples of the 3 groups were measured by ELISA. The expression of CD62p in 2 apheresis experimental groups was measured by flow cytometry. The differences between the two groups were compared. Results The levels of VEGF, TGF-β1 and PDGF, which were （410.95 ± 95.07） pg/ml, （91.15 ± 19.50） ng/ml and （ 12.60 ± 2.06 ） ng/ml, respectively, in group 1 were markedly higher than those in the plasma control group, which were 149.09 ± 28.11, 37.38± 10.73 and 3.28 ± 0.79, rcspeetively （ P 〈 0.01 ）, while the levels in group 1 were significantly lower than that in group 2, which were 495.16 ± 63.49,110.33± 19.06 and 16.96 ±2.71, respectively （ P 〈 0.05 ）. Platelet counts in these samples showed a positive correlation to the levels of VEGF, TGF-β1 and PDGF growth factors. Conclusion There are high expressions of VEGF, TGF-β1 and PDGF in apheresis platelets, and activation of platelets could promote the production and release of these growth factors.

关 键 词：单采血小板 血浆 血小板活化 血管内皮细胞生长因子 转化生长因子-β1 血小板源性生长因子 血小板计数 CD62P ELISA 流武细胞术 组织创面 

分 类 号：R331.143[医药卫生—人体生理学] R457.1[医药卫生—基础医学]