尿蛋白分离中微流控芯片的制作与电渗流速度检测的研究  

On fabrication of microfluidic chip and detection of electroosmotic flow velocity in separating clinical urine protein

作　　者：孙惠芹[1] 刘南平[2] 王翔[1] 刘志兰[3] 李峰[4] 

机构地区：[1]天津职业大学,天津300402 [2]天津师范大学,天津300387 [3]天津第二医院,天津300000 [4]上海交通大学,上海200030

出　　处：《天津师范大学学报（自然科学版）》2009年第1期39-41,47,共4页Journal of Tianjin Normal University：Natural Science Edition

摘　　要：提出了微流控芯片应用于尿蛋白分离中的芯片制作与电渗流速度检测方法,讨论了用SG4009匀胶铬版玻璃制作微流控芯片过程中提高芯片质量的方法;采用以CCD为图像传感器的微通道尺寸的检测方法和以Rh123中性分子为标定物的直接测定电渗流速度的方法进行尿蛋白分离.结果表明,该法可提高临床的诊断率.The methods of fabricating the microfluidic chip and detecting the eleetroosmotic flow velocity in separating clinical urine protein are proposed. In the process of fabricating microfluidic chip using SG4009 Cr photoresist layer glass, the methods of improving the quality of the chip are discussed. The sizes of microchannels are measured by using CCD as image sensor, and the electroosmotic flow velocity is detected directly by using Rh123 as calibration. Applying microfluidic chip to urine protein separation, the clinical diagnosis rate improved.

关 键 词：微流控芯片 尿蛋白 电渗流 Rh123 

分 类 号：TP274.5[自动化与计算机技术—检测技术与自动化装置]