一种强寄生病原真菌的分离方法——毛细管打孔单孢分离法  

Isolating Strongly Parasitic Fungi by Single-spore Isolation Aided with Capillary Stiletto

作　　者：董娟华[1] 罗丽[1] 王彩霞[1] 冷伟锋[1] 李桂舫[1] 李保华[1] 

机构地区：[1]青岛农业大学农学与植物保护学院,山东青岛266109

出　　处：《中国农学通报》2009年第3期210-212,共3页Chinese Agricultural Science Bulletin

基　　金：国家自然科技资源共享平台项目"林业微生物菌种资源标准化整理;整合及共享试点"(No.2005DKA21207);公益性行业(农业)科研专项"苹果轮纹病菌及其抗性资源信息库建立"(No.NYHYZX07-055)

摘　　要：毛细管打孔单孢分离是将真菌孢子涂布在2%琼脂板上,显微镜下找到单孢后,用内径1mm毛细管直接打孔,然后挑取带有单孢的琼脂饼培养。毛细管打孔简化了单孢分离的操作过程,提高了单孢分离的速度和成功率,特别适合强寄生真菌的分离培养。用于梨黑星病菌、苹果褐斑病菌和樱桃褐斑病菌的分离培养,成功率达60%以上。Steps to isolate strongly parasitic fungi by single-spore were： smear the fungi spores on to the surface of 2% water agar, find a single-spore under microscope, separate the spore by press a capillary （with the diameter of 1 mm） into the agar, pick the piece of agar wafer with the spore and then transferred to corresponding medium. This method simplified isolation procedures, improved isolation speed and success rate, and was especially suitable for isolating strongly parasitic fungus. The method was used to isolate Venturia nashicola, Diplocarpon mall and Cercospora circumscissa and more than 60% single-spores isolated with this method developed to colonies.

关 键 词：单孢分离 分离培养 强寄生真菌 

分 类 号：S432.1[农业科学—植物病理学]