银杏叶总黄酮延缓细胞衰老的实验研究  

Experimental Study on Effect of Total Flavone of Ginkgo biloba on Delaying Cell Senescence

作　　者：宋小平[1] 陈志武[2] 方安宁[1] 方佩斐 

机构地区：[1]安徽医学高等专科学校生物教研室,安徽合肥230061 [2]安徽医科大学药理学教研室,安徽合肥230032

出　　处：《中药材》2009年第1期100-102,共3页Journal of Chinese Medicinal Materials

基　　金：安徽省教育厅自然科学研究科目(KJ2007B036)

摘　　要：目的:研究银杏叶总黄酮(total flavone of Ginkgo biloba,FG)延缓人胚肺二倍体成纤维细胞衰老的作用。方法:采用含FG的大鼠血清对人胚肺二倍体成纤维细胞2BS细胞株进行处理,观察2BS细胞寿命;采用荧光实时定量PCR法检测P16基因mRNA的表达。结果:银杏叶总黄酮(FG)能够延长2BS细胞的传代寿命,下调2BS细胞P16基因mRNA的表达。结论:银杏叶总黄酮可通过抑制P16基因表达而延缓细胞衰老。Objective： To investigate the mechanism of delaying the senescence of human diploid fibroblast （2BS）by total flavone of Ginkgo biloba（FG）. Methods： The drug sera of FG was used to treat the 2BS. The population doublings of 2BS cells were observed, the mRNA expression of P16 gene was determined by fluorescence real-time quantitative PCR. Results： FG significantly extended the population doublings of 2BS cells, and decreased the expression of P16 mRNA. Conclusion： FG can delay the senescence of cells by inhibiting the P16 gene expression.

关 键 词：银杏叶总黄酮 细胞衰老 人胚肺二倍体成纤维细胞 荧光实时定量PCR 

分 类 号：R285.5[医药卫生—中药学]