大理白族自治州乙型肝炎病毒基因型分布研究  

Distribution of hepatitis B virus genotypes in Dali Bai Autonomous Prefecture

作　　者：任来峰[1,2] 张烜榕[1] 申元英[1] 王涛[1] 陈钟[1] 周勇[1] 李威[1] 

机构地区：[1]大理学院,云南大理671000 [2]山西医科大学汾阳学院,山西汾阳032200

出　　处：《中国热带医学》2009年第3期428-429,458,共3页China Tropical Medicine

基　　金：国家自然科学基金资助项目(编号30560136)

摘　　要：目的了解大理地区白族人群感染乙型肝炎病毒(HBV)基因型的分布。方法对202份乙型肝炎表面抗原(HBsAg)阳性血清采用基因型特异性引物-PCR法进行基因型检测。结果202份血清标本中100份(49.5%)HBV基因分型阳性。其中,B基因型41份,占41%;C基因型25份,占25%;B+C混合基因型34份,占34%。在HBV基因型B、C、B+C中HBeAg阳性率分别为:68.3%(28/41)、40.0%(10/25)、58.8%(20/34),B、C基因型比较HBeAg阳性率有显著性差异(χ2=5.089,P=0.024)。结论大理地区HBV基因型主要为B基因型、C基因型和B+C混合基因型,最显著的特点是B+C混合基因型感染占很大比例。Objectives To investigate the distribution of hepatitis B virus genotypes among people with HBV-infection in DaLi Bai Autonomous Prefecture. Methods A total of 202 serum samples with positive HBsAg were collected for detection of HBV genotypes by nested polymerase chain reaction （PCR） with HBV genotype-specific primers. Results Of 202 serum samples,100（49.5%） were HBV genotypes positive; 41 were genotype B （41%）,25 were genotype C（25%） and 34 were mixed genotype （B＋C）（34%）. The positive rates of HBeAg among genotype B, genotype C, mixed genotype （B＋C） were 68.3% （28/41）,40.0%（ 10/25 ）, 58.8% （20/34）respectively. Positive rates of HBeAg were significantly different in genotype B and genotype C （x^2=5.089,P=0.024）. Conclusions genotype B, mixed genotype （B＋C） and genotype C are major HBV genotypes among people with HBV in DaLi; A significant feature is the very high rates of mixed genotype （B＋C） in patients with HBV.

关 键 词：乙型肝炎病毒 基因型 聚合酶链反应 

分 类 号：R373.21[医药卫生—病原生物学]