乳化异氟醚强化停跳液对成年大鼠心肌作用的研究  

Cardioprotective effects of emulsified isoflurane strengthened cardioplegia on ischemia-reperfusion injury in isolated rat hearts

作　　者：胡强[1] 王娟[2] 常昕[1] 龙村[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京阜外心血管病医院体外循环科,北京100037 [2]安徽省淮北市矿工总医院麻醉科,安徽淮北235000

出　　处：《实用临床医药杂志》2009年第1期5-8,16,共5页Journal of Clinical Medicine in Practice

摘　　要：目的探讨乳化异氟醚对成年大鼠离体心脏心肌缺血-再灌注(I/R)损伤的影响。方法32只成年SD大鼠随机分成4组:STH液组,脂肪乳组,乳化异氟醚H组(5 mmol/L),乳化异氟醚L组(1 mmol/L)。每组均接受4℃停跳后,在30℃下接受60 min停灌,37℃复灌60 min。测定各组心脏机械功能,冠脉流出液中心肌肌钙蛋白I(cTnI)的含量,取左室心肌测定丙二醛(MDA)含量及电镜检查。结果乳化异氟醚L组和脂肪乳组可显著增强左室发展压,减少冠脉流出液中cTnI的含量(P<0.01),减少心肌丙二醛(MDA)含量;心肌细胞电镜观察显示两组心肌细胞损伤轻于STH液组。H组乳化异氟醚显著减低左室发展压,增加冠脉流出液中中cTnI的含量(P<0.01),增加心肌丙二醛(MDA)含量;心肌细胞电镜观察显示心肌细胞损伤明显高于其余各组。结论低浓度乳化异氟醚强化停跳液可改善心脏机械功能,促进离体成年大鼠心脏心肌缺血-再灌注(I/R)后血流动力学恢复,对成年大鼠心肌缺血-再灌注损伤有保护作用。Objective To investigate the cardioprotective effects of emulsified isoflurane on ischemia-reperfusion injury. Methods 32 Sprague-Dawley rats were randomly divided into the following four groups： control group one with STH solution; group two with 30% fat emulsion; high -dose group with emulsified isoflurane 5 mmol/L; low-dose group with emulsified isoflurane 17 mmol/L. Every group were reperfused 60rain at 37 ℃ after these hearts stopped beating and were stopped perfusing for 60min. Left ventricular development pressure（LVDP） and maximum rate of pressure increase, minimum rate of pressure decrease （dp/dtmin）. Coronary flow （CF） and myocardial cell structure were obtained and myocardial ultrastructure was examined under electronic microscope. Results The contents of MDA and cTnI in group intralipid and low-dose group with emulsified isoflurane were much lower than those of control group （P 〈 0.01 ）. The LVDP was strengthened obviously in group intralipid and low-dose group with emulsified isoflurane. The contents of MDA and cTnI in high-dose group with emulsified isoflurane were much highter than those. of other groups （P 〈 0.01）. The myocardial ultrastructural changes were significant in these four groups and there were significant edema and severe damage in myocardium in high-dose group with emulsified isoflurane; but it was not so severe in 30 % of fat emulsion group and low-dose emulsified isoflurane group. Conclusion Low-dose emulsifiedisoflurane strengthened eardioplegia solution was beneficial on ischemia-reperfusion injury in isolated rat hearts.

关 键 词：乳化异氟醚 脂肪乳 缺血-再灌注 心肌保护 

分 类 号：R542.2[医药卫生—心血管疾病]