腈水解酶在羧酸合成中的研究进展  

Nitrilase mediated hydrolysis of nitriles in organic acid synthesis

作　　者：何玉财[1,2] 许建和[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]中国科学院青岛生物能源与过程研究所,青岛266101

出　　处：《生物加工过程》2009年第1期7-12,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20506037;20672037);华东理工大学生物反应器工程国家重点实验室开放课题资助项目(2008004)

摘　　要：有机羧酸是有机合成中的重要中间体,用腈水解酶催化有机腈实现有机羧酸的合成不仅具有反应条件温和、污染少和易处理等优点,而且更重要的是能实现一般化学法所不能达到的高度化学、区域和立体选择性。综述了腈水解酶的来源、特性和作用机理,介绍了腈水解酶在有机合成中的研究进展以及该酶在工业上的应用前景。Organic carboxylic acids are important intermediates in organic synthesis. By using nitrilase as biocatalyst, hydrolysis of nitriles to yield carboxylic acids can be performed under mild conditions with low pollution and without waste disposal problem. Additionally, it can also provide high chemoselectivity, regioselectivity and enantioselectivity, and they can＇t be achieved by conventional organic synthesis. Sources, properties, and the mechanism of the nitrilase were reviewed. The research progress of nitrilases in organic synthesis was introduced, and the potential application of nitrilases in industry were summarized.

关 键 词：生物催化 有机羧酸 腈水解酶 有机合成 

分 类 号：Q556[生物学—生物化学]