胸腺基质淋巴细胞生成素基因的克隆及表达条件的优化  

Cloning and Optimization Expression of Thymic Stromal Lymphopoietin

作　　者：唐蓓[1] 李艳[1] 吴丹[1] 袁均林[1] 丁书茂[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室,湖北武汉430079

出　　处：《化学与生物工程》2009年第2期25-27,共3页Chemistry & Bioengineering

基　　金：国家科技支撑计划资助项目(2006BAI19B05;2006BAJ02A10-1)

摘　　要：将BamHI和EcoRI酶切过的胸腺基质淋巴细胞生成素(TSLP)基因片段插入载体pET28a,然后转化大肠杆菌BL21(DE3),在IPTG的诱导下实现了TSLP基因在大肠杆菌中的表达。对表达条件进行了优化,在TB+M9(1∶1)培养基中,37℃下使用终浓度0.4 mmol·L-1的IPTG诱导3 h,重组TSLP的表达量可占全菌蛋白的18.6%左右,比优化前提高了约15%。In order to express the thymic stromal lymphopoietin （TSLP） in E. coli, after digestion with BamHI and EcoRI, the fragment was inserted into pET28a, and the recombinant plasmid was named as pET28a-TSLP. The recombinant plasmid was transformed into BL21 （DE3）. With the inducement of IPTG, the recombinant pro-TSLP was successfully expressed. According to changing the induction temperature and inducer concentration, the recombinant pro-TSLP could achieve 18.6% of total protein in TB＋M9 （1 ： 1） culture medium, which was induced with 0.4 mmol· L^-1 IPTG at 37℃ for 3 h.

关 键 词：胸腺基质淋巴细胞生成素 克隆 表达 优化 

分 类 号：Q78[生物学—分子生物学]