射干药材的HPLC指纹图谱研究  

Study on HPLC fingerprint of Rhizoma Belamcandae

作　　者：张媛媛[1,2] 秦民坚[1,2] 束盼[1,2] 黎路[1] 

机构地区：[1]中国药科大学中药资源学教研室 [2]中国药科大学现代中药教育部重点实验室,南京210038

出　　处：《药学与临床研究》2009年第1期18-21,共4页Pharmaceutical and Clinical Research

摘　　要：目的:研究不同产地射干药材的HPLC指纹图谱,为控制射干药材质量提供依据。方法:采用Agilent Zorbax SB-C_(18)色谱柱(4.6 mm×250 mm,5μm);乙腈-0.4%冰醋酸水溶液为流动相,梯度洗脱;流速1 mL·min^(-1);检测波长269nm结果:15个不同产地射干药材的指纹图谱有14个共有峰,通过与对照品的保留时间比较,确定了其中7个峰结论:虽然不同产地药材指纹图谱具有一定差异,HPLC指纹图谱仍可以用作射干药材的质量控制。Objective ：To study the HPLC fingerprints and establish a sensitive and specific method for the quality control of Rhizoma Belamcandae from different origins. Methods： The samples were separated on an Agilent Zorbax SB-C18 column （4.6 mm ×250 mm, 5 um） with the mobile phase consisting of acetonitrile and 0.4% acetic acid water solution by gradient elution. The flow rate was l mL.min^-1 ; wavelength was 269 nm. Results： There were 14 common peaks in the HPLC fingerprints of 15 samples. By comparision of the retention time of chemical standards, 7 peaks were identified. Conclusions： Although the HPLC fingerprints of the Rhizoma Belamcandae from different origins were varied slightly, the established HPLC fingerprints can be used for the quality control of Rhizoma Belamcandae.

关 键 词：射干 高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]