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作 者:张慧英[1] 田夏红[1] 冯锐[2] 刘爱花[3]
机构地区:[1]广西大学农学院,广西南宁530005 [2]广西农业科学院,广西南宁530007 [3]广西农业职业技术学院,广西南宁530007
出 处:《安徽农业科学》2009年第1期99-100,108,共3页Journal of Anhui Agricultural Sciences
基 金:广西自然科学基金资助项目(桂科基0236005);广西教育厅资助项目(桂教科研[2002]316)
摘 要:[目的]探讨不同科之间DNA导入后的性状变异情况,并从中筛选优良稳定变异株系。[方法]利用花粉管通道法将大豆总DNA导入普通玉米自交系7313,逐代选择,至玉米田间性状、籽粒颜色、形状、果穗轴色等均稳定,对筛选出的变异株系果穗籽粒进行粗蛋白、醇溶蛋白、谷蛋白和油分含量的测定和比较。[结果]株系26h-4-3的籽粒粗蛋白、醇溶蛋白、谷蛋白和油分含量与对照相比差异都达到显著或极显著水平,在D3和D4代的提高幅度分别为10.34%和26.70%、6.58%和6.28%、15.09%和70.34%5、5.82%和51.52%;株系26h-3-1的各项指标与对照相比也有极大提高,在D3和D4代的提高幅度分别为5.67%和21.63%、1.91%和2.31%、10.85%和62.27%2、2.49%和9.67%。[结论]26h-4-3和26h-3-1变异株系的粗蛋白、醇溶蛋白、谷蛋白和油分含量都稳定提高,是符合高蛋白育种目标的优良变异株系。[Objective] The experiment was aimed to explore character variation between different families after DNA introduction and select variant plants with good stability.[Method] The method of pollen-tube-pathway was used to introduce total DNA of soybean into normal maize inbred line 7313 for selecting generation by generation.When field characters of maize,grain colors,grain traits and panicle axis colors were stable,the crude protein,gliadin,glutelin and oil content of grains which were selected from variant strains were detected and compared.[Result] The grain crude protein,gliadin,glutelin and oil content of line 26h-4-3 were significantly different from these of control treatment.The increments of D3 and D4 generation were 10.34% and 26.70%,6.58% and 6.28%,15.09% and 70.34%,55.82% and 51.52% respectively.All indexes of line 26h-3-1 were also higher than these of control treatment and the increments of D3 and D4 generation were 5.67% and 21.63%,1.91% and 2.31%,10.85% and 62.27%,22.49% and 9.67%.[Conclusion] The crude protein,gliadin,glutelin and oil content of variant line 26h-4-3 and 26h-3-1 were stable,so variant line 26h-4-3 and 26h-3-1 were excellent variant strains which satisfied the requirement of high protein breeding.
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