端粒酶RNA亚基的shRNA对细胞端粒酶活性影响的研究  

Study on the Effect of shRNA of chTR on Telomerase Activity of Cell

作　　者：张哲[1,2] 明宇[1,2] 于聪[3] 张玉静[1,2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学人兽共患病研究所,吉林长春130062 [3]中国科学院长春应用化学研究所,吉林长春130022

出　　处：《安徽农业科学》2009年第1期147-149,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探讨端粒酶RNA亚基(chTR)的短发RNA(shRNA)shRNA对MDCC-MSB1细胞端粒酶活性的影响。[方法]设计合成chTRshRNA并构建表达载体,转染MDCC-MSB1细胞,应用改良TRAP法检测端粒酶活性。[结果]与对照组相比,转染24 h后,各组端粒酶活性变化不明显;转染48 h后,端粒酶活性均显著地下降,且针对模板区设计的干扰载体pSi-chTR-sh1转染后抑制效果最明显。[结论]chTRshRNA表达载体能够有效地抑制MDCC-MSB1细胞的端粒酶活性。[Objective] The aim of this study was to investigate the inhibitory effects of shRNA of chTR on telomerase activity in MDCC-MSB1 cells.[Method] The artificially designed and synthesized short hairpin RNA（shRNA） of chicken telomerase RNA（chTR） was used to construct the expression vector that was subsequently transfected to MDCC-MSB1 cells,then the telomerase activity of these cells was measured by improved TRAP method.[Result] Compared to the CK group,the telomerase activities in the treatment cells showed no obvious decrease in 24 h after transfection,while decreased remarkably 48 h later,among which in the cells transfected by interference vector pSi-chTR-sh1 presented the most obvious inhibitory effects.[Conclusion] This vector could effectively inhibit the telomerase activities in MDCC-MSB1 cells.

关 键 词：端粒酶 端粒酶RNA SHRNA 

分 类 号：S188[农业科学—农业基础科学]