牛粪堆肥中添加抑菌剂对大肠杆菌杀灭效果的研究  

Sterilization Effects of Bacterial Inhibitor on Escherichia coli in Cattle Manure Compost

作　　者：敖日格乐[1] 王纯洁[1] 于俊娥[1] 斯木吉德[1] 吴美云[1] 程佳[1] 陈昆[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018

出　　处：《安徽农业科学》2009年第1期243-244,共2页Journal of Anhui Agricultural Sciences

基　　金：内蒙古自然科学基金重点项目(200607010403);国家科技支撑计划项目(2006BAD04A15)

摘　　要：[目的]研究牛粪堆肥中添加抑菌剂(石灰氮)对大肠杆菌的杀灭效果,为牛粪的无害化处理提供理论依据。[方法]试验组添加2.0%的抑菌剂,对照组不加抑菌剂,分别在不同的温度(20、303、75、06、0℃)条件下进行堆肥试验,确定适宜堆肥温度;并在30℃条件下添加不同剂量(0、2.0%、2.5%、3.0%)的抑菌剂,确定抑菌剂的最适添加量。[结果]305、0、60℃是杀灭大肠杆菌较理想的温度;在30℃条件下,添加2.5%和3.0%的抑菌剂可在堆肥48 h时检测不到大肠杆菌,说明为达到杀灭牛粪中大肠杆菌的目的至少要在牛粪堆肥中添加2.5%的抑菌剂。[结论]在堆肥(特别是冬季堆肥)中添加石灰氮对提高牛粪中大肠杆菌杀灭效果具有重要意义。[Objective] The aim of this study was to explore the sterilization effects on Escherichia coli by adding bacterial inhibitor（CaCN2） during the process of cattle manure composting so as to provide a theoretical basis for cattle manure harmless treatment.[Method] Both experimental groups supplemented with 2.0% bacterial inhibitor and control groups without bacterial inhibitor were cultured at different temperatures（20,30,37,50,60 ℃） to determine the optimal composing temperature.Under 30 ℃,different doses（0,2.0%,2.5%,3.0%） of bacterial inhibitor were added into the compost to obtain the optimal bacterial inhibitor addition dose.[Result] 30,50 and 60 ℃ were ideal temperatures for sterilization of E.coli.Under 30 ℃,E.coli couldn＇t be detected in 2.5% dose group and 3.0% dose group after culture for 48 h,demonstrating no less than 2.5% bacterial inhibitor should be added.[Conclusion] It has an important significance to enhance the sterilization effects on E.coli by adding CaCN2 into cattle manure compost especially in winter.

关 键 词：抑菌剂 牛粪 堆肥 大肠杆菌 

分 类 号：S141.4[农业科学—肥料学]