抗绿脓杆菌外毒素—A血浆（马）抗体效价检测方法的探讨  

Comparative Study of Methods for Titre Deterimination of Equine Anti-Pseudomonas areruginosa Exotoxin A Plasma

作　　者：刘哈霆[1] 陈孝婷[1] 王盟[1] 秦刚 

机构地区：[1]卫生部兰州生物制品研究所,兰州730046

出　　处：《中国人兽共患病杂志》1998年第1期17-19,共3页Chinese Journal of Zoonoses

摘　　要：目的：为了探讨在生产条件下采用哪种方法来检测抗绿脓杆菌外毒素—A血浆（马）的抗体效价更为可行。方法：本文对小鼠中和试验，双向琼脂扩散试验及胶乳凝集试验进行了比较研究。结果实验显示，小鼠中和试验和双向琼脂扩散试验特异性良好，结果稳定，但前者灵敏度比后者更高。而胶乳凝集试验由于胶乳抗原不易保存，其结果稳定性较差。结论：我们认为小鼠中和试验适用于大规模生产时采用，双向琼脂扩散可作为初步效价的检测。In order to approach which method is the most suitable one in the titre detection of equine anti-Pseudomonas areruginosa exotoxin A plasma. METHOD:Mouse neutralization test. Double immunodiffusion test and latex agglutination reaction were compared RESULTS:The results showed that the mouse neutralization test and double immunodiffusion test have the advantages of good-special and reliable-resuirs,but sensitivity of the former is higher than one of the latter. The results from latex agglutination were unreliable due to latex anigen was preserved uneasy. CONCLUSION: So we sugest that the mouse netralization was best suited for detecting the titre,double immunodiffusion test was available.

关 键 词：绿脓杆菌 外毒素A 抗体效价 小鼠 中和试验 

分 类 号：R378.991[医药卫生—病原生物学] R446.62[医药卫生—基础医学]