气相色谱-质谱联用技术研究半夏厚朴汤配伍对挥发油成分的影响  

Effects of Different Compatibility of Banxiahoupu Decoction on Changes of Volatile Oils by GC-MS

作　　者：徐群[1] 武露凌[2] 王彩萍[1] 汪水娟[1] 孔令东[1] 

机构地区：[1]南京大学医药生物技术国家重点实验室,江苏南京210093 [2]江苏省体育科学研究所,江苏南京210033

出　　处：《中国实验方剂学杂志》2009年第2期5-10,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金重大研究计划中医药学几个关键问题的现代研究项目(90409009)

摘　　要：目的:分析半夏厚朴汤及相关配伍样品挥发油成分种类和相对含量等变化,探讨半夏厚朴汤配伍与其挥发油成分变化的关系及规律。方法:利用气相色谱-质谱(GC-MS)法分离检测复方半夏厚朴汤、单味药厚朴、紫苏叶、生姜、及厚朴与紫苏叶、厚朴与生姜、紫苏叶与生姜等配伍样品挥发油成分。结果:各样品具有数目不同的挥发性成分,其中半夏厚朴汤54个、厚朴44个、紫苏叶15个、生姜53个、及配伍厚朴与紫苏叶26个、厚朴与生姜51个、紫苏叶与生姜75个。厚朴与紫苏叶、厚朴与生姜及紫苏叶与生姜等配伍后成分主要来源于分别为厚朴、生姜。复方成分种类主要源于3个单味药材的加和,而其挥发油成分主要来源于生姜。结论:半夏厚朴汤及配伍前后挥发性成分的数目及含量有一定的差异性,表明在煎煮过程中相关样品中挥发性成分溶解性发生变化或相互间产生了化学反应。Objective：To study the species and relative contents of volatile oils in the well-known Banxiahoupu Decoction and its related combinations and to explore the connections between the different compatibility of Banxiahoupu Decoctions and changes of volatile oils. Method： GC-MS method was used to analyze seven kinds of Banxiahoupu combinations： Banxiahoupu Decoction, Magnolia officinalis, Perilla frutescens, Zingiber officinale, Magnolia officinalis with Perilla frutescens, Magnolia officinalis with Zingibcr officinale, Perilla frutescens with Zingiber officinale. Result： Differences were found in volatile oils： 54 oil in Banxiahoupu Decoction, 44 in Magnolia officinalis, 15 in Perilla frutescens, 53 in Zingiber officinale, 26 in compatibility of Magnolia officinalis and Perilla frutescens, 51 in compatibility of Magnolia officinalis and Zingiber officinale, 75 in compatibility P. frutescens and Zingiber officinale. Magnolia officinalis and Zingiber officinale contributed mostly to components of compatibility of Magnolia officinalis and Perilla frutescens, Magnolia officinalis and Zingiber officinale, as well as Perilla frutescens and Zingiber officinale, respectively. The volatile oils of Banxiahoupu decoction were a summation of components of Magnolia officinalis, Perilla frutescens and Zingiber officinale, and of which most components were identical with those in Zingiber officinale. Conclusion： There isa great difference in species and relative contents of volatile oils beforo and-after compatibility, indicating the deliquescent of volatile components changed or certain chemical reactions taken place in the decoction process.

关 键 词：半夏厚朴汤 配伍 挥发油 气相色谱-质谱法 总离子流图 

分 类 号：R284.1[医药卫生—中药学]