两种工艺制备的参芪扶正注射液对荷S180小鼠网状内皮系统吞噬廓清能力的影响  

Effects of Shenqi Fuzheng Injections Prepared by Two Different Crafts on the Ability of Reticuloendothelial System in S_(180)-bearing Mice

作　　者：丁治国[1] 史晓光[1] 李兰芳[2] 汪唐顺[1] 陈振宙[1] 杨庆[2] 张建松[1] 叶广铎[1] 李乃卿[1] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2009年第2期36-38,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：教育部产学研综合项目(2006D90504018)

摘　　要：目的：比较两种工艺制备的参芪扶正注射液对荷S180小鼠网状内皮系统吞噬廓清能力、抑制肿瘤能力的影响。方法：42只荷瘤小鼠随机分成5组，分别为对照组、现工艺参芪扶正注射液高剂量组（8g·kg^-1）和临床等效剂量组（4g·kg^-1）、原工艺参芪扶正注射液高剂量组（8g·kg^-1）和临床等效剂量组（4g·kg^-1）。ip给药，1次，d，12d后以碳廓清法测网状内皮系统吞噬指数K，然后处死小鼠，称取体重、肿瘤、肝、脾及胸腺重量。结果：4个给药组瘤重和肿瘤指数皆低于对照组，除原工艺的低剂量组外，与对照组的差异均有统计学意义（P〈0．01～P〈0．05），同一剂量两种工艺制剂之间差异无统计学意义；现工艺的2个剂量组吞噬指数皆高于对照组，与对照组的差异有统计学意义（P〈0．01-P〈0．05）；4g·kg^-1剂量组，现工艺组吞噬指数显著高于原工艺组（P〈0．05）；4个给药组脾指数皆高于对照组（P〈0．05），肝指数及胸腺指数无显著性差异；同一剂量两种工艺制剂比较无显著性差异。结论：在临床等效剂量（4g·kg^-1），现工艺制备的参芪扶正注射液可显著提高荷瘤小鼠的网状内皮系统吞噬廓清能力，效果优于原工艺；同时可有效延缓肿瘤生长，提高脾指数，与原工艺无明显差异。Objective：To compare effects of Shenqi Fuzheng Injections prepared by two different crafts on the ability of reticuloendothelial system, the ability to restrain tumor and the protection to some important organs in S180- bearing mice. Methods： 42 tumor-bearing mice were randomly divided into five groups：the model group, the high dose group（8 g·kg^-1）and the clinical equivalent dose group（4 g·kg^-1） of Shenqi Fuzheng Injection on current craft, the high dose group （8 g· kg^-1） and the clinical equivalent dose group （4 g· kg^-1） of Shenqi Fuzheng Injection on previous craft. The model group was given physiological saline by intraperitoneal injection once a day, while the other groups were given different Shenqi Fuzheng Injections in the same way. Twelve days later, blood samples were collected from the mice and phagocytic index K was determined, Then the mice were sacrificed, the weights of body, tumor, spleen, liver and thymus gland were collected. Results ： The weights of tumors and tamor indexes in four Shenqi Fuzheng Injection groups were lower than the model group, and the difference between groups of Shenqi Fuzheng Injection on current craft and the model group was significant（ P 〈 0.01 - 0.05）. However the groups of the current craft and the previous one had no significant difference; phagocytic index K in four Shenqi Fuzheng Injection groups were higher than that in the model group, and the difference between groups of Shenqi Fuzheng Injection on current craft and the model groups was significant （ P 〈 0.01 0.05） ; and in 4 g· kg^- 1 groups, the current group was better than the previous one （ P 〈 0.05）. The spleen indexes in four Shenqi Fuzheng Injection groups were higher than that in the model group （P 〈 0.05）, the liver indexes and the thymus gland one have no significant difference. Conclusion： In clinical equivalent dose （4 g·kg^-1）, the ability of reticuloendothelial system in Shenqi Fuzheng Injection on current craft was better than the mode

关 键 词：参芪扶正注射液 制备工艺 S180细胞 网状内皮系统 

分 类 号：R285.5[医药卫生—中药学]