硒对氟中毒鸡肝肾GSH-Px活性及其mRNA表达的影响  

Effects of Dietary Selenium on the Activity of GSH-Px and the Expression of GSH-Px mRNA in Liver and Kidney of Fluorositic Chicks

作　　者：于倩[1] 周良生[1,2] 左楠[1] 贾海燕[1] 李金敏[1] 王俊杰[1] 李术[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]黑河市爱辉区畜牧兽医局,黑龙江黑河164300

出　　处：《中国家禽》2009年第3期11-13,共3页China Poultry

摘　　要：为探讨硒对氟中毒鸡肝肾组织GSH-Px活性及其mRNA表达的影响,选取180只7日龄海兰褐蛋鸡,随机分为3组,每组60只。Ⅰ组:正常对照组,饲喂全价日粮;Ⅱ组:氟中毒组,在正常日粮中添加氟化钠(NaF),使日粮中氟含量为1000mg/kg日粮;Ⅲ组:加硒组,在氟中毒组日粮基础上添加亚硒酸钠(Na2SeO3),使日粮中硒含量为4mg/kg日粮。试验期为90d,每隔30d采样,进行肝脏和肾脏谷胱甘肽过氧化物酶(GSH-Px)活性的检测以及GSH-Px mRNA转录水平的测定。结果表明加硒组肝脏和肾脏GSH-Px活性和GSH-Px mRNA转录水平在各时间点均高于氟中毒组(P<0.05)。说明日粮中加硒可在一定程度上颉颃氟所致鸡肝肾组织脂质过氧化作用。To investigate effects of adding selenium to diets on the activity of GSH-Px and the transcription level of GSH-Px mRNA in liver and kidney of fluorositic chicks,180 7-day-old chicks were randomly divided into 3 groups,60 chickens each group. Group Ⅰ was the control,group Ⅱ were fed the diets containing 1 000 mg/kg of fluorine as sodium fluoride,and group Ⅲ 1 000 mg/kg of sodium fluoride and 4 mg/kg sodium selenite,for 90 days. The activities of GSH-Px and the transcription level of GSH-Px mRNA in liver and kidney were determined at every other 30 day. The results showed as follows：During each stage,the activity of GSH-Px and transcription level of GSH-Px mRNA in livers and kidneys in group Ⅲ were higher than that in the group Ⅱ （P〈0.05）. It can be conclude that selenium in some degree can antagonism and defense the damages to the capacity of anti-oxidation of livers and kidneys by fluorosis.

关 键 词：硒 氟 鸡 抗氧化 

分 类 号：S865.9[农业科学—野生动物驯养] S852.4[农业科学—畜牧兽医]