一株鸵鸟胚源新城疫病毒生物学特征和遗传发生分析  

Molecular Characterization and Phylogenetic Tree of An Isolate of Newcastle Disease Virus from Dead Ostrich Embryo

作　　者：焦库华[1] 高以明[2] 张敬友[3] 王水明[2] 柯家法[3] 李超美[2] 朱雪良[2] 张扬[3] 严继宁[2] 黄立业[2] 任晓进[2] 吴艳涛[1] 刘文博[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏出入境检验检疫局,江苏南京210001 [3]南京出入境检验检疫局,江苏南京210001

出　　处：《中国家禽》2009年第3期22-25,共4页China Poultry

基　　金：国家质检总局科技计划项目(2007KJ03);江苏省高校自然科学基础研究面上项目(07KJB230140);江苏省教育厅指导计划项目(06KJD230211)

摘　　要：从死亡的鸵鸟胚胎中分离到1株新城疫病毒毒株,经毒力检测为强毒株,鸡胚平均死亡时间为45h,IVPI为2.90,IPCI为1.88。RT-PCR扩增出融合蛋白(F)基因,测定核苷酸序列并进行遗传发生分析和酶切位点分析。对基因编码的氨基酸序列进行了推导,该毒株的蛋白酶裂解激活位点附近的氨基酸序列为112R(K)RQKR↓F117,为强毒特征序列,然而该毒株并未引起该鸵鸟群发病。根据绘制的系统进化发生树和F基因上3种限制性内切酶(RE)位点分布,确定该毒株均为基因Ⅶ型。该病毒与在我国鸡群、鹅群同期流行的毒株有很高的同源性。A isolate of Newcastle disease virus from dead ostrich embryo was characterized. The isolate was a virulent strain based on the MDT,ICPI and IVPI. The genes of fusion protein were sequenced. The amino acid sequence between 112 to 117 were 112R （K）RQKR ↓ F117. The restriction enzyme cleavage site mapping of the fusion protein gene between nucleotides 334 and 1 682 and sequence analysis between nucleotides 47 and 420 were studied. Both methods revealed the isolate belonged to genotype Ⅶ c. The characteristics of the isolate were as same as other isolates of this genotype from geese and chickens in recent years.

关 键 词：鸵鸟 新城疫病毒 遗传发生树 

分 类 号：S941.41[农业科学—水产养殖] S858.315.3[农业科学—水产科学]