高磁性与无磁性趋磁细菌的获得及磁小体释放技术研究  

Cultivating technology of high-magnetic and non-magnetic AMB-1

作　　者：张宇红[1,2] 肖天[1] 

机构地区：[1]中国科学院海洋研究所海洋生态与环境科学重点实验室,山东青岛266071 [2]中国科学院研究生院,北京100864

出　　处：《海洋科学》2009年第2期1-4,10,共5页Marine Sciences

基　　金：中国科学院海外杰出学者基金项目(2003-1-5,2006-1-15);中国科学院创新团队国际合作伙伴计划项目;人类前沿科学研究计划项目(HFSP,RGP0035/2004-C)

摘　　要：利用特殊的磁富集传代法获得高磁性和无磁性AMB-1菌株。通过透射电子显微镜(TEM)观察和Cmag值测定,对这两种菌株进行了验证。优选了条件,使高磁性AMB-1菌株在培养192 h后出现自溶现象,磁小体从细胞内释放出来。Magnetospirillum sp. AMB-1 of high-magnetic and non-magnetic were obtained by special enrichment and inoculation method. After that this two kind of bacteria were validated by transmission electron microscope （TEM） and were measured Cmag. Furthermore, under optimizing growth conditions and cultivating time, AMB-1 of high-magnetic lysed and magnetosomes released from cells. As a result, magnetosomes were gotten conveniently by such method.

关 键 词：AMB-1 纳米磁小体 磁小体释放 

分 类 号：Q93[生物学—微生物学]