海洋毒素大田软海绵酸完全抗原的制备与分析  

Preparation and analysis of complete antigen for okadaic acid

作　　者：卢士英[1] 周玉[1] 任洪林[1] 霍方珍[1] 潘风光[1] 柳增善[1] 于师宇 于光[3] 

机构地区：[1]人兽共患病教育部重点实验室,吉林大学人兽共患病研究所,吉林长春130062 [2]福清出入境检验检疫局,福建福清350300 [3]吉林出入境检验检疫局,吉林长春130062

出　　处：《海洋科学》2009年第2期46-49,54,共5页Marine Sciences

基　　金：国家自然科学基金资助项目(30671762);国家质检总局资助项目(2003IK044,2008IK003);吉林大学农学部博士启动基金资助项目(4305050102C8)

摘　　要：为了建立大田软海绵酸(Okadaic acid,OA)的免疫学检测方法,利用活泼酯法将OA分子中的羧基与载体蛋白上的氨基偶联,人工制备OA的免疫抗原(OA-IgG)和检测抗原(OA-BSA),经电泳、动物实验及ELISA法进行了鉴定,完全抗原的偶联成功,为利用杂交瘤技术制备OA的单克隆抗体和海产品OA免疫学检测方法奠定了良好的基础。In order to construct the immunological detection method for okadaic acid, the carboxyl group of OA molecule is coupled to amino group of carrier protein IgG and BSA using active ester method. It certificates that the man-made prepared OA immunizing antigen and detecting antigen are successfully coupled by electrophoresis, animal experiment and ELISA, These establish favourable foundation for preparation the antibody against OA using the technique of Hybridoma and immunological method detecting OA from marine food.

关 键 词：大田软海绵酸(okadaic ACID OA) 活泼酯法 抗原偶联 

分 类 号：X56[环境科学与工程—环境工程]