青龙衣不同萃取部位抗肿瘤活性研究  

Antitumor Activity of Different Solvent Fractions of Qinglongyi Extracts

作　　者：曲中原[1,2] 邹翔[2] 崔兰[2] 胡国军[2] 季宇彬[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]哈尔滨商业大学生命科学与环境科学研究中心,国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076

出　　处：《上海中医药杂志》2009年第1期87-90,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30600816);教育部高等学校博士点学科专项基金资助项目(20060240001)

摘　　要：目的研究青龙衣乙醇提取物及不同溶剂萃取部位对肿瘤细胞的体外抑制作用,筛选青龙衣抗肿瘤活性部位。方法采用四甲基偶氮唑蓝(MTT)法,选用4种细胞株(SGC-7901,HepG-2,HCT-8,Capan-2)进行体外实验,对青龙衣不同萃取部位的抗肿瘤活性进行筛选。结果青龙衣乙醇提取物、石油醚部位、氯仿部位、乙酸乙酯部位对肿瘤细胞均有不同程度的抑制作用。其中青龙衣乙醇提取物对SGC-7901,HepG-2,HCT-8,Capan-2的IC_(50)分别为47.18,31.12,35.45,27.81 mg/L;石油醚部位对SGC-7901, HepG-2,HCT-8,Capan-2的IC_(50)分别为56.72,57.14,25.96,15.21 mg/L;氯仿部位对SGC-7901,HepG-2,HCT-8,Capan-2的IC_(50)分别为32.08,29.59,32.47,60.72 mg/L;乙酸乙酯部位对SGC-7901,HepG-2,HCT-8,Capan-2的IC_(50)分别为35.79,35.48,24.48, 25.46 mg/L。正丁醇部位和水溶性部位对各肿瘤细胞无抑制作用。结论青龙衣的乙醇提取物、石油醚部位、氯仿部位、乙酸乙酯部位有一定的细胞毒作用,石油醚部位、氯仿部位、乙酸乙酯部位初步确定为青龙衣抗肿瘤活性部位。Objective To estimate the antitumor activity of Qinglongyi alcohol extract and different solvent fractions and screen the antitumor active fractions in vitro. Methods MTT assay was applied to screen the antitumor solvent fractions on different tumor cell lines in vitro. Results Qinglongyi alcohol extract, petroleum e- ther part, chloroform part and ethyl acetate part had inhibitory effect on different tumor cell lines. The IC50 of Qinglongyi alcohol extract against SGC-7901, HepG-2, I-ICT-8 and Capan-2 was 47.18, 31.12, 35.45, 27.81 mg/L. The IC5o of petroleum ether part against SGC-7901, HepG-2, HCT-8 and Capan-2 was 56.72, 57.14, 25.96 and 15.21 mg/L respectively. The IC50 of chloroform part against SGC-7901, HepG-2, HCT-8 and Capan-2 was 32.08, 29.59, 32.47 and 60.72 mg/L respectively. The IC50 of ethyl acetate part against SGC-7901, HepG-2, HCT-8 and Capan-2 was 35.79, 35.48, 24.48 and 25.46 mg/L respectively. Butanol part and the water-soluble part had no effect on different tumor cell lines. Conclusion Qinglongyi alcohol extract, petroleum ether part, chloroform part and ethyl acetate part have considerable cytotoxin effect. Petroleum ether part, chloroform part and ethyl acetate part are considered as antitumor active parts of Qinglongyi.

关 键 词：青龙衣 萃取部位 抗肿瘤活性 

分 类 号：R285.5[医药卫生—中药学]