浙江温州地区腹泻患儿腺病毒的检测及分型  

Detection and typing of adenoviruses in feces of infants with diarrhea in Wenzhou

作　　者：黄燕燕[1] 郑晓群[2] 彭颖[1] 华科[1] 陈新宇[2] 

机构地区：[1]温州医学院检验学院生命科学学院,浙江温州325035 [2]温州医学院附属第二医院检验科,浙江温州325027

出　　处：《中国卫生检验杂志》2009年第1期24-25,共2页Chinese Journal of Health Laboratory Technology

基　　金：温州市科技发展计划基金项目(Y2006A033)

摘　　要：目的:了解浙江温州地区婴幼儿腹泻患者不同型别腺病毒的感染情况。方法:根据Gene Bank中腺病毒六邻体基因序列设计一对通用引物,建立PCR方法检测婴幼儿腹泻标本中腺病毒DNA,并对阳性标本进行PCR产物测序分析。结果:建立了快速、特异地检测婴幼儿腹泻标本中腺病毒DNA的PCR技术;127份婴幼儿腹泻标本中腺病毒DNA阳性4份,阳性率为3.15%(4/127);经PCR产物直接测序分析,3份为Ad3型,阳性率为2.36%(3/127),1份为Ad7型,阳性率为0.79%(1/127)。结论:PCR技术结合PCR产物直接测序的方法,使分析具有敏感、特异等优点,适用于腹泻标本中腺病毒的检测及分型;引起浙江温州地区婴幼儿腹泻的腺病毒主要为Ad3型和Ad7型。Objective：To study the adenoviruses infection in infants with diarrhea in Wenzhou,Zhejiang.Methods：According to hexon gene of adenovirus,one prime pair was designed as universal primes and applied to detect adenovirus DNA by PCR.127 fecal specimens from diarrhea infants were tested,and positive specimens were sequenced.Results：A rapid and specific PCR assay for detection adenovirus was set up.The positive rate of adenovirus DNA in fecal specimens was 3.15%（4/127）.And the positive rate of adenovirus type 3（Ad3） was 2.36%（3/127）,the positive rate of adenovirus type7（Ad7） was 0.79%（1/127）.Conclusion：PCR combined with sequence analysis is more sensitive and specific,which could be used for identifying types of adenovirus in clinical specimens.Ad3 and Ad7 are important pathogens causing infant diarrhea in Wenzhou.

关 键 词：腺病毒 腹泻 PCR 分型 

分 类 号：R722.132[医药卫生—儿科]