耐甲氧西林金黄色葡萄球菌基因分型及耐药性研究  

Genotyping and antibiotic resistance of methicillin-resistant Staphylococcus aureus

作　　者：林辉[1] 金春光[1] 郑剑[1] 闫鹏[1] 章丹阳[1] 沈玄艺[1] 

机构地区：[1]浙江省宁波市疾病预防控制中心,浙江宁波315010

出　　处：《中国卫生检验杂志》2009年第1期34-35,62,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:研究医院重症监护病房(ICU)、烧伤病房环境中耐甲氧西林金黄色葡萄球菌(MRSA)的分子流行病学特征。方法:采用脉冲场凝胶电泳(PFGE)分型技术对MRSA进行同源性分析,K-B纸片扩散法进行药敏试验。结果:28株MRSA的PFGE指纹图谱显示7个基因型(A-G),以A型(8株)和E型(6株)为主,分别来自烧伤病房和ICU。其对青霉素、氨苄青霉素、阿莫西林、苯唑西林、红霉素、环丙沙星、诺氟沙星、氧氟沙星等8种抗生素耐药率均为100%,对头孢西丁、庆大霉素、四环素、氯霉素、复方新诺明耐药率分别为96.4%、67.9%、64.3%、42.9%、14.3%,仅对万古霉素100%敏感。结论:MRSA耐药性较为严重,其PFGE基因分型阐述了烧伤病房和ICU分别存在MRSA单克隆流行趋势。Objective：To investigate the characteristic of the molecular epidemiology and antibiotic resistance of Methicillin-resistant Staphylococcus aureus（MRSA） isolated from intense care unit（ICU） and burns wards.Methods：Pulsed-field gel electrophoresis（PFGE） and Kirby-Bauey method were used to analyze the homology and drug-resistance of MRSA strains,respectively.Results：There were seven genotypes（A-G） in 28 strains of MRSA,and genotype A（eight strains） and E（six strains） were isolated from burnt wards and ICU.To penicillin,ampicillin,amoxicillin,oxacillin,illotycin,ciprofloxacin,norfloxacin,ofloxacin,the resistance rates of MRSA were all 100%.And to cefoxitin,cidomycin,ambramycin,amphemycin,trimethoprim-sulfamethoxazole,the resistance rates of MRSA were 96.4%,67.9%,64.3%,42.9%,14.3%,respectively.But the sensitivity of MRSA to vancomycin was 100%.Conclusion：The resistance of antibiotic is a serious problem in MRSA,and result of PFGE elucidates that monoclone prevalent trend may exist in ICU and burns wards.

关 键 词：耐甲氧西林金黄色葡萄球菌 脉冲场凝胶电泳 基因分型 耐药性 

分 类 号：R378.11[医药卫生—病原生物学]