日化产品霉腐微生物的研究  

Research of mildew and bacteria in daily chemical products

作　　者：王春华[1] 谢小保[1] 邱晓颖[1,2] 曾海燕[1] 黄小茉[1,2] 廖浩华 欧阳友生[1] 陈仪本[1] 

机构地区：[1]广东省菌种保藏与应用重点实验室广东省微生物研究所,广州510070 [2]广东迪美生物技术有限公司,广州510070

出　　处：《中国卫生检验杂志》2009年第1期36-38,共3页Chinese Journal of Health Laboratory Technology

基　　金：广东省科技攻关项目(2005B10401014)

摘　　要：目的:为日化产品霉腐微生物污染的治理及其防腐体系的构建提供科学依据。方法:采集腐败变质的日化产品样品进行霉腐微生物培养。细菌鉴定按照《常见细菌系统鉴定手册》及16S rRNA序列和生物梅里埃API系统执行,真菌鉴定按《真菌鉴定手册》及BIOLOG鉴定系统执行。结果:日化产品中腐败霉变的微生物53.6%为革兰阴性细菌,其中的主要类群为假单胞菌、洋葱伯克氏菌、肠杆菌和嗜麦芽窄食单胞菌。革兰阳性细菌以芽孢杆菌(17.6%)和葡萄球菌(3.2%)为主。真菌主要为曲霉属和青霉属。乳状(包括乳液)日化产品中分离出的微生物最多,占总比例的36.0%。导致各个区域日化产品霉腐的微生物最主要的为G+芽孢杆菌、G-杆菌和曲霉属。其中除中部外,其它区域的G-杆菌比例皆在50%以上。结论:导致日化产品的霉腐微生物种类较多,应根据其种类、理化性状及地域不同进行治理。Objective：To provide the scientific basis for preventing the contamination of daily chemical products by mildew and bacteria and the construction of preservation system.Methods：Collected daily chemical product specimens according to different regions,different categories and their chemical and physical properties.Then cultivated different microorganisms using special media and characterized bacteria and fungus according to Manual of Systematic and Determinative Bacteriology,16S rRNA genes and API System,Manual for the Identification of Fungi and BIOLOG System,respectively.Results：Most of the bacterial strains were Gram-negative（53.6%）,the dominant strains of which were Pseudomonas,Burkholderia cepacia,Enterobacter and Stenotrophomonas maltophilia.The dominant strains of Gram positive bacteria were spore-producing rods（17.6%） and staphylococcus（3.6%）.Fungi were Aspergillus and Penicillium.36.0% microorganisms were acquired from milky daily chemical products.In all the regions the dominant strains were mainly spore-producing rods,Gram-negative rods and Aspergillus.Besides,Gram-negative rods were above 50% in most regions.Conclusion：Daily chemical products were contaminated by many microorganisms.So their categories,regions and chemical and physical properties should be considered when preventing the contamination and constructing the preservation system.

关 键 词：日化产品 微生物 污染 

分 类 号：R117[医药卫生—公共卫生与预防医学]