牛Pbx-1基因的电子克隆及生物信息学分析  

In Silico Cloning and Bioinformatics Analysis of the Cattle Pbx-1 Gene

作　　者：刘卉玲[1,2] 崔群维[3] 吴国良[1,2] 范睿[1,2] 蔡亚非[1,2] 王根林[3] 

机构地区：[1]安徽师范大学生物环境与生态安全安徽省高校重点实验室,安徽芜湖241000 [2]安徽师范大学重要生物资源保护与利用研究重点实验室,安徽芜湖241000 [3]南京农业大学动物科技学院,江苏南京210095

出　　处：《激光生物学报》2009年第1期17-23,共7页Acta Laser Biology Sinica

基　　金："十一五"国家科技支撑计划项目(2006BAD04A01;2006BAD04A12);芜湖市科技计划重点项目(2008620);安徽省自然科学基金项目(050410201)

摘　　要：新基因全长cDNA序列很难获得,但电子克隆却提供了基因克隆的一种策略。利用小鼠Pbx-1基因编码序列(NM_183355)为种子序列进行电子克隆获得牛Pbx-1基因完整编码序列。然后,用生物信息学方法分析了牛的Pbx-1基因的结构,密码子偏性和氨基酸的同源性等。结果表明:该基因cDNA全长1 754 bp,无内含子,最大开放阅读框1 305 bp,编码434个氨基酸,预测其编码的蛋白分子量为47 189.5 D a,与小鼠的同源性为81%。It is not easy to obtain the full-length cDNA sequence of a novel gene, however, in silico cloning provides a strategy for gene cloning. The complete coding sequence of cattle Pbx-1 gene was obtained by using mouse Pbx-1 gene coding sequence （NM_183355） as seeded sequence in silico cloning. Afterwards, the structure and codon adaptation of Pbx-1, and amino acid homology of Pbx-1 protein in cattle was analyzed by using bioinformatics methods. The results show that the cDNA of cattle Pbx-1 gene is 1 754 bp with no introns, containing a 1 305 bp open reading frame, and encodes 434 amino acids with a molecular weight of 47 189.5 Dalton, and the cattle Pbx-1 gene has 81% homology with the mouse counterpart.

关 键 词：牛Pbx-1基因 电子克隆 生物信息学 

分 类 号：Q785[生物学—分子生物学]