糖尿病大鼠肾脏病变及其SnoN蛋白表达的变化  

The Renal Lesions and SnoN Protein Expression in Kidney Tissue of Rats with Type 2 Diabetes

作　　者：崔龙[1] 刘瑞霞[1] 李晓颖[1] 石明隽[1] 肖瑛[1] 郭兵[1] 张国忠[1] 

机构地区：[1]贵阳医学院病理生理学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2009年第1期16-20,共5页Journal of Guiyang Medical College

基　　金：贵州省科技攻关项目(编号:黔科字S[2007]1033号);贵州省高层次人才科研条件特助经费资助项目(编号:No.TZJF-2007年-44号);贵州省科技基金资助项目(编号:黔科合J字[2008]2167号)

摘　　要：目的:复制2型糖尿病动物模型,并观察其肾脏病变及SnoN蛋白表达的变化。方法:SD大鼠随机分为正常对照组(NC)、高脂高糖组(HG)。高脂、高糖饮食12周后又随机分为高脂高糖对照组(HC)、糖尿病组(DM)。采用高脂、高糖饮食12周加小剂量链脲佐菌素注射(30 mg/kg)的方法复制2型糖尿病动物模型,成模大鼠随机分别于糖尿病8周(DM8)、12周(DM12)和16周(DM16)处死;Western印迹法检测肾皮质SnoN蛋白的水平;生物化学方法测血糖、血清胰岛素水平、甘油三酯、总胆固醇、血肌酐及24 h尿蛋白;光镜检查肾组织形态学改变。结果:DM组大鼠出现高血糖、高血脂、肾功能和肾组织形态学改变;HG组血清胰岛素水平、甘油三酯和总胆固醇明显升高,血糖与NC组相比差异无统计学意义;Western印迹检测发现DM8组肾组织中SnoN蛋白的表达明显低于NC组,且随病程进展逐渐减少,至DM16时减至NC组的64.20%。结论:成功复制了2型糖尿病动物模型;SnoN蛋白的表达变化可能参与了2型糖尿病肾脏病变的发生、发展过程。Objective： To establish type 2 diabetic rat model and observe its renal lesions and SnoN protein expression. Methods： Sprague-Dawley rats were divided into normal control, high-fat and high-sucrose groups. High-fat and high-sucrose groups were then divided into high-fat and high-sucrose eontMs and diabetes mellitus groups after taking high-fat and high-sucrose diet for 12 weeks. The diabetic rats were injected with low dose of streptozotoein （30 mg/kg） and were killed at the 20^th, 24^th, and 28^th weeks respectively. The SnoN proteins in renal cortex were detected with Western blotting. Blood glucose, serum insulin concentration, trigly-eeride, total cholesterol, serum creatinine and 24 h urine protein were examined with biochemistry methods. The renal morphology on HE stained sections was checked with light microscope. Results： Diabetic rats showed hyperglycaemia, hyperlipemia, kidney morphological lesion and renal function changes. High-sucrose control group showed hyperinsulinemia and hyperlipemia, but the blood glucose was normal. Western blotting results showed that the expression of SnoN in diabetic renal tissues significantly decreased （P 〈 0.01 ）. Conclusions： Rat models of type 2 diabetes mellitus have been established successfully which can be used as a diabetic nephropathy model. The change of SnoN protein expression may play a role in the mechanisms of type 2 diabetic nephropathy.

关 键 词：糖尿病 非胰岛素依赖型 糖尿病肾病 大鼠 Sprague—Dawley 转录共抑制因子SnoN 

分 类 号：R587.1[医药卫生—内分泌]