放射性鼻黏膜损伤后组织纤维化重塑的特点  

Fibrotic remodeling of nasal mucosa in rat model after radiation injury

作　　者：肖芒[1] 汤建国[1] 孙晓南[2] 姜秀文[1] 罗宝珍[1] 赵丽娜[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院耳鼻咽喉头颈外科浙江省生物治疗重点实验室,杭州310016 [2]浙江大学医学院附属邵逸夫医院放疗科,杭州310016

出　　处：《中华耳鼻咽喉头颈外科杂志》2009年第2期130-135,共6页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：基金项目：浙江省自然科学基金资助项目（Y205073）

摘　　要：目的探讨鼻黏膜纤维化在放射性鼻黏膜损伤中的作用。方法清洁级SD大鼠72只，雄性，随机分为两组，空白对照组36只，予以假照射；辐射损伤组36只，予以X射线局部照射，总辐射剂量40Gy。损伤组分别于辐射完成后1周、2周、4周、8周、3个月和6个月（每个时间点各6只大鼠）配对处死大鼠，取出中鼻甲予以扫描和透射电镜观察，苏木素-伊红（hematoxylin-eosin，HE）染色；阿辛蓝-过碘酸-希夫（alcian blue-periodic acid—Schif，AB—PAS）染色，Masson三色胶原染色（Masson trichrome，MT）法染色，观察鼻黏膜组织病理变化；化学比色法测定鼻黏膜羟脯氨酸（Uyp）的含量。结果辐射损伤后，早期（4周内）主要以急性炎性反应为特点，4周后修复开始，6个月时修复基本结束。受照后1周，大鼠鼻黏膜中出现胶原沉积，胶原沉积量随着时间的推移而进行性增多。结论照射能引起鼻黏膜组织一系列的病理改变，鼻黏膜纤维化可能是放射性鼻黏膜损伤迁延不愈的重要原因之一。Objective To investigate the role of nasal mucosa fibrosis on radiation induced nasal mucosa injury. Methods Seventy two male rats were randomly divided into two groups, control group and irradiation injured group （ radiation dose were 40 Gy） ; the rats were killed 1 week, 2 weeks, 4 weeks, 8 weeks, 3 months and 6 months after the finish of radiation. The middle turbinates of the animals were removed. The pathological change of the nasal mucosa were observed with scanning electron microscope, transmission electron microscope, hematoxylin and eosin （ HE）, alcian blue-periodic acid-Schif （ AB-PAS）, and Masson Trichrome （MT）. The Hyp content in nasal mucosa was measured with chemo-chromatometry. Results After radiation, the pathological characteristics in early stage （within 4 weeks ） was acute inflammatory reaction. The repair of nasal mucosa started 4 weeks after radiation, lasted to 6 months. The deposition of collagen in nasal mucosa could be found 1 week after irradiation and increased gradually. Conclusion Irradiation could induce a serials of pathological changes on nasal mucosa. The nasal mucosa fibrosis may be one of the reasons of persistent irradiation induced nasal mucosa iniury.

关 键 词：辐射损伤 实验性 大鼠 鼻黏膜 纤维化 

分 类 号：R686[医药卫生—骨科学]