氯化两面针碱诱导人肝癌细胞SMMC-7721凋亡的研究  

Nitidine chloride-induced apoptosis of human hepatoma cell SMMC-7721

作　　者：李丹妮[1] 刘华钢[1] 刘丽敏[1] 刘林[1] 梁霜[1] 

机构地区：[1]广西医科大学药学院药理学教研室,广西南宁530021

出　　处：《西安交通大学学报（医学版）》2009年第1期40-43,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：广西科学研究与技术开发计划资助项目(No.桂科攻0630002-2E)~~

摘　　要：目的研究氯化两面针碱诱导人肝癌细胞SMMC-7721凋亡的作用。方法采用噻唑蓝(MTT)比色法检测人肝癌细胞SMMC-7721生长活力;Hoechst33258核染色,观察细胞形态学变化;流式细胞仪检测细胞周期及细胞凋亡率。结果氯化两面针碱作用于细胞后,在体外呈浓度、时间依赖性显著抑制SMMC-7721细胞的生长,作用24、48、72h的半数抑制浓度(IC50)分别为(19.5±1.8)、(3.8±0.2)、(2.8±0.1)mg/L;Hoechst33258染色后,可见核染色质凝集,凋亡细胞呈致密浓染,与对照组相比,氯化两面针碱处理后凋亡细胞比例增加;流式细胞仪检测到G2/M细胞比例增加,G0/G1、S期细胞比例下降。药物作用于SMMC-7721细胞后,随浓度增加细胞凋亡率增加。结论氯化两面针碱对SMMC-7721细胞有明显的生长抑制作用,可能与改变细胞周期并诱导细胞凋亡有关。Objective To study the effect of nitidine chloride in inducing apoptosis of human hepatoma cell SMMC-7721. Methods Cell viability was measured by MTT assay in human hepatoma cell line SMMC-7721. Morphological changes of cell nuclei were observed by Hoechst 33258 staining. Flow cytometry was performed to measure the cell cycle and cell apoptosis. Results Proliferation of SMMC-7721 cells was significantly inhibited by nitidine chloride in concentration- and time- dependent manners in vitro, the 50% inhibition concentration （IC50） value at 24, 48, and72 h being （19.5±1.8）, （3.8±0.2） and （2.8±0.1）mg/L, respectively. The chromatine concentration and apoptotic cells with high concentrations were observed by Hoechst 33258 staining. The percentage of apoptotic cells increased in nitidine chloride -treated groups compared with that in the control group. The percentage of ceils in G2/M phase increased and that of G0/G1, S phases was decreased according to flow cytometry determination. After treatment with nitidine chloride, apoptosis rate of SMMC-7721 cells increased with the increase of concentration. Conclusion Nitidine chloride significantly inhibits the growth of SMMC-7721 cells. The antihepatoma mechanism of SMMC-7721 cells may be associated with the changes of cell cycle and inducing cell apoptosis.

关 键 词：氯化两面针碱 SMMC-7721细胞 G2/M期阻滞 细胞凋亡 肝癌 

分 类 号：R282.71[医药卫生—中药学]