复方斑蝥注射液抗小鼠肝癌H_(22)肿瘤作用及机理的初步研究  

Effect of Compound Cantharis Injection on the Protein Expression of Fas and in H_(22) Tumor Cell

作　　者：张建武[1] 闵冬雨[2] 唐建平[3] 张贵林[4] 

机构地区：[1]川北医学院基础医学院药理教研室,四川南充637100 [2]徐州医学院基础医学院药理教研室,江苏徐州221002 [3]川北医学院基础医学院机能实验中心,四川南充637100 [4]贵阳医学院基础医学院药理教研室,贵州贵阳550004

出　　处：《川北医学院学报》2009年第1期9-12,共4页Journal of North Sichuan Medical College

摘　　要：目的研究复方斑蝥注射液(Compound Cantharis Injection,CCI)的抗肿瘤作用,并观察CCI对小鼠H22肿瘤细胞Fas蛋白表达的影响。方法(1)通过小鼠腋皮下接种H22肿瘤细胞,观察CCI对H22小鼠的抑瘤作用。(2)用免疫组织化学的方法检测该药对小鼠H22肿瘤细胞Fas蛋白表达的影响。结果(1)实验证实,与模型组比较,CCI有显著的抑制小鼠H22实体瘤的作用(p<0.05),该药高剂量对小鼠H22抑制率为34.83%。(2)免疫组织化学结果显示,与模型组比较,CCI能上调Fas蛋白表达(p<0.05)。结论CCI可能通过上调Fas蛋白表达,诱导肿瘤细胞凋亡而抑制H22肿瘤细胞的生长。Objective To investigate antitumor effect of compound traditional Chinese of cantharis injection （CCI）, and to observe the protein expression of Fas in H22 tumor cells by treatment of CCI. Methods （ 1 ） By inoculating subcutaneously with the tumor cells of H22, then the inhibitory rate of CCI on H22 in vivo was observed. （2） By immunohistoehemistry staining the Fas protein expression in H22 tumor cells was detected. Results （1）CCI could obviously inhibit the growth of H22 in vivo（p 〈 0.05）. The experiment found out that the rates of tumor inhibition of the big dosage against H22 in vivo all was 34.83%. （2）CCI could obviously increase the Fas protein expression in H22 tumor cells （p 〈 O. 05 ）. Conclusions （ 1 ） CCI can obviously inhibit the growth of H22 in vivo. （2） CCI can inhibit the growth of H22 in vivo by obviously increasing the Fas protein expression in H22 tumor cells.

关 键 词：复方斑蝥注射液 H22 FAS 蛋白表达 

分 类 号：R969.4[医药卫生—药理学]