不同阶段小鼠囊胚玻璃化冷冻保存效果观察  

Observation on vitrification of mouse blastocysts at different development stages

作　　者：付敏[1] 章晓梅[2] 李蓉[1] 李永刚[2] 

机构地区：[1]北京大学深圳医院生殖医学科,深圳518036 [2]云南省第一人民医院生殖遗传科,昆明650032

出　　处：《生殖医学杂志》2009年第1期44-46,共3页Journal of Reproductive Medicine

摘　　要：目的探讨合适玻璃化冷冻保存的小鼠囊胚发育阶段。方法对575枚小鼠初级囊胚和577枚扩张囊胚分别采用六组玻璃化溶液进行玻璃化冷冻,比较不同阶段囊胚的冷冻保存效果。结果各组初级囊胚存活率和孵出率均高于扩张囊胚。除ES35组外,各组初级囊胚存活率均显著高于扩张囊胚(P<0. 05) ; EDS35 、 ESF20和EDSF20组的初级囊胚的孵出率均显著高于扩张囊胚(P<0. 05)。结论初级囊胚较扩张囊胚更适合玻璃化冷冻保存。Objectives： To investigate the suitable stage for the vitrification of mouse blastocysts. Methods. Five hundred and seventy-five early blastocysts and 577 expanded blastocysts were frozen with six different vitrification solutions. The survival rate and hatching rate of the thawed blastoeysts were compared. Results： The survival rate and hatching rate of all early blastocysts were higher than those of expanded blastocysts. Except for those in EG35 group, the early blastocysts had a significantly higher survival rate than expanded blastocysts （P〈0.05）. In EDS35, ESF20 and EDSF20 groups, the hatching rate of early blastocysts was significantly higher than that of expanded blastocysts （P〈0.05）. Conclusion： The blastocysts at early stage are more suitable for vitrification.

关 键 词：初级囊胚 扩张囊胚 冷冻保存 玻璃化 小鼠 

分 类 号：R318.52[医药卫生—生物医学工程]