PCR检测在真菌性角膜溃疡中的诊断价值  

Applied polymerase chain reaction detecting fungal corneal ulcer

作　　者：路西林[1] 薛会敏[1] 赵朝贤[2] 杨建峰 

机构地区：[1]邯郸市第三医院检验科,056001 [2]河北工程大学医学院生化教研室,056002 [3]邯郸市邯钢医院检验科,056001

出　　处：《疑难病杂志》2009年第2期80-81,共2页Chinese Journal of Difficult and Complicated Cases

基　　金：邯郸市科技攻关指令性计划项目(No.0828108054-2)

摘　　要：目的探讨聚合酶链反应(PCR)快速检测在真菌性角膜溃疡诊断中的价值。方法以源于医学条件致病性真菌18srRNA基因保守区的一对寡核苷酸序列为通用引物,对临床拟诊为真菌性角膜溃疡的36例标本进行PCR检测,并与培养方法进行对比。结果在400bp处出现DNA扩增带者为阳性。36份临床标本的真菌培养阳性率为58.3%,而经PCR扩增阳性率为86.1%,PCR扩增准确性为72.2%,敏感性为100.0%,特异性为33.3%。结论真菌通用引物进行PCR反应检测真菌性角膜溃疡速度快、阳性率高,有助于真菌性角膜溃疡的快速诊断。Objective To establish a method for rapid detection of clinical suspect fungal corneal ulcer by polymerase chain reaction（PCR）. Methods A pair of oligonucleotide sequences, which was bases on the conserved region of 18srRNA shared by medically important conditioned fungal, was used as the general primers to amplify the DNAs from clinical suspect fungal corneal ulcer in a PCR assay, and the result was contrasted with culture. Results A 400bp specific DNA product was successfully amplified. The positive rate of fungi culture among 36 clinical specimens was 58.3 %, while that of PCR amplification was 86.1%. In addition, the accuracy of PCR method in this study was 72.2%, the sensitivity was 100%, and the specialty was 33.3 %. Conclusion PCR with the general primers is suitable for rapid detection of fhngal corneal ulcer because of quickness and high positive rate.

关 键 词：聚合酶链反应 角膜溃疡 真菌性 诊断 

分 类 号：R450[医药卫生—治疗学] R772.21[医药卫生—临床医学]