栀子提取物对单纯疱疹病毒1型感染小鼠VP16mRNA和IFN-γmRNA的影响  

Effect of the Gardenia Extracts-T9 on Viral Replication and IFN-γ mRNA in Herpes Simplex Virus Type-1 Infected Mice Brains

作　　者：时宇静[1] 黄洋[1] 郭姗姗[1] 姜晶[2] 苏丹[1] 赵晔[1] 高英杰[1] 崔晓兰[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]北京大学航天临床医学院,北京100049

出　　处：《病毒学报》2009年第1期41-46,共6页Chinese Journal of Virology

基　　金：国家自然科学基金面上项目(30672646);国家十一五科技支撑项目(2006BAI08B04-02)

摘　　要：采用RT-PCR技术,分别在单纯疱疹病毒1型感染BALB/C小鼠4、7、10、14、21天时,检测小鼠脑内VP16 mRNA和IFN-γ表达的变化,考察栀子提取物T9对病毒复制和宿主免疫的影响。结果显示,栀子提取物T9各给药组VP16 mRNA相对表达量在同一时间内均低于病毒对照组,其中T9低剂量组VP16 mRNA相对表达量在感染后第21天明显下降,T9高剂量组VP16 mRNA相对表达量随时间变化呈现持续降低状态;栀子提取物T9各给药组在除第4天外的其他时间点IFN-γmRNA相对表达量均高于病毒对照组,其中T9低剂量组IFN-γ mRNA相对表达量在感染后第4天至第14天随时间变化而明显增高,之后表达量略有下降,T9高剂量组IFN-γ mRNA相对表达量随时间变化呈现持续升高状态。结果提示,栀子提取物T9可能通过上调单纯疱疹病毒感染小鼠脑内IFN-γ mRNA相对表达量,来抑制单纯疱疹病毒在小鼠脑内的复制。RT-PCR was used to detect expression level of VP16 mRNA and IFN-γ mRNA in Herpes simplex virus type-1 infected mice brains at 4th day, 7th day, 10th day, 14th day, 21st day post infection and investigate the effects of the Gardenia extracts-T9 on viral replication and host immunity. The results showed that expression of VP16 mRNA in Gardenia extracts-T9 high dose and low dose group were both lower than that in virus control group at same time point. Relative VP16 mRNA expression in low dose group decreased at 21st day and relative VP16 rnRNA expression in high dose group decreased continuously. Relative expression of IFN-γ/mRNA in high dose and low dose groups were both higher than that in virus control group at all time point except the 4th day. IFN-γ mRNA in low dose group increased from the 4th day till the 14th day, and after the 14th day, the expression decreased slightly. Relative IFN-γ mRNA in high dose group maintained increasing from 4th day till 21st day. Base on these results, we conclude that Gardenia extracts-T9 might exert the inhibition effect of viral replication by upregulating expression of IFN-γ mRNA.

关 键 词：栀子提取物T9 单纯疱疹病毒1型 RT—PCR IFN-Γ 病毒复制 

分 类 号：R373.11[医药卫生—病原生物学]