黄瓜绿斑驳花叶病毒辽宁分离物全基因组序列测定  

Complete Genomic Sequence of a Watermelon Isolate of Cucumber Green Mottle Mosaic Virus in Northern China

作　　者：陈红运[1,2] 林石明[1] 陈青[1] 赵文军[2] 廖富荣[1] 陈洪俊[2] 朱水芳[2] 

机构地区：[1]厦门出入境检验检疫局,厦门361012 [2]中国检验检疫科学研究院,北京100029

出　　处：《病毒学报》2009年第1期68-72,共5页Chinese Journal of Virology

基　　金：国家科技支撑计划项目(2006BAD08A13);国家质检总局项目(2007IK253,2008IK264)

摘　　要：以感病组织总RNA为模板,采用RT-PCR方法扩增并测定黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)辽宁分离物(CGMMV-LN)的基因组全序列。CGMMV-LN基因组全长6 422 nt,5'非编码区(noncoding region,NCR)和3'NCR分别为59 nt和175 nt。CGMMV-LN编码的4个蛋白依次是186 kD和129kD的复制酶,29 kD的移动蛋白和17.4 kD的外壳蛋白。CGMMV-LN与其他4个CGMMV分离物基因组核苷酸序列同源性为97.6%～99.3%,与同属其他3种病毒基因组核苷酸序列同源性仅为61.7%～62.8%。基于186kD复制酶和外壳蛋白氨基酸序列的同源树显示:侵染葫芦科作物的烟草花叶病毒属病毒可分为2个亚组,亚组I包括所有CGMMV分离物,亚组II包括Kyuri绿斑驳花叶病毒(Kyuri green mottle mosaic virus,KGMMV)、黄瓜果实斑驳花叶病毒(Cucumber fruit mottle mosaic virus,CFMMV)和小西葫芦绿斑驳花叶病毒(Zucchini greenmottle mosaic virus,ZGMMV)。The complete genomic sequence of a watermelon isolate of Cucumber green mottle mosaic virus （CGMMV-LN） in Liaoning province was determined and compared with other cucurbit-infecting tobamo- viruses. The genomic RNA of CGMMV-LN comprised 6 422 nt, and 5＂- and 3＂- noncoding regions consisted of 59 nt and 175 nt, respectively. The encoded four proteins were two replicase proteins of 186 kD and 129 kD, move protein of 29 kD and coat protein of 17.4 kD. The alignment results of complete nucleotide sequence showed that CGMMV-LN shared identities of 97.6%- 99.3% with four other CGMMV isolates, but only shared identities of 61.7%- 62.8%o with three other tobamoviruses. Homology trees generated from replicase proteins of 186 kD and coat proteins suggested that cucurbit-infecting tobamoviruses could be separated into two subgroups： subgroup I comprising all the isolates of CGMMV and subgroup II comprising Cucumber fruit mottle mosaic virus, Kyuri green mottle mosaic virus and Zucchini green mottle mosaic virus.

关 键 词：黄瓜绿斑驳花叶病毒 烟草花叶病毒属 序列分析 

分 类 号：S432.4[农业科学—植物病理学]