盐生植物海马齿中与K^＋离子胁迫有关的基因功能初步鉴定  

The Primary Function Identification of a K^+-Tolerance Related Gene from a Halophyte S. portulacastrum L.

作　　者：刘钢[1,2] 段瑞军[1] 郭建春[1] 

机构地区：[1]中国热带农业科学院生物技术研究所,海口571101 [2]海南大学农学院,儋州571737

出　　处：《基因组学与应用生物学》2009年第1期22-26,共5页Genomics and Applied Biology

基　　金：国家973计划(2007CB108903);中国热带农业科学院(RKy0725);中央级公益性科研院所基本科研项目(ITBBYB072)资助

摘　　要：盐生植物海马齿常年生长在海边沙地,能忍耐高盐干旱。本研究对从海马齿植物盐胁迫下的差减文库(SSH文库)中分离的3个全长基因SRTG152-Ⅰ、SRTG152-Ⅱ和SRTG152-Ⅲ(GenBank:FJ457924,FJ457925和FJ457926)进行了微生物表达和耐盐分析。3个全长基因分别插入pET-22b(+)表达载体,经过1mmol/L的IPTG在37℃下诱导3h,它们均获得了成功表达。耐盐性功能分析表明,3个基因对Ca2+、Mg2+和Na+离子没有抗性,而在900mmol/L高浓度K+离子的胁迫下与对照相比赋予菌株一定的K+离子抗性。所以,我们初步推测这3个全长基因SRTG152-Ⅰ、SRTG152-Ⅱ和SRTG152-Ⅲ是一类与K+离子的抗性相关的基因。The Halophyte S. portulacastrum L. living in seaside is a kind of high-salt and drought-tolerant plant. In this report, the expression and the salt-tolerant analysis of the three genes SRTG152- Ⅰ , SRTG152- Ⅱ and SRTG152-Ⅲ（GenBank： FJ457924, FJ457925 and FJ457926） isolated from salt stressed SSH bank have been researched in E. coli by inserting them into a vector pET-22b（＋）. The expressing proteins were obtained aiter inducing 3 h with 1 mmol/L IPTG at 37℃. The analysis of their salt tolerances showed that all the three genes did not resist to Ca^2＋, Mg^2＋ and Na^＋, but showed K^＋ resistance under 900 mmol/L K^＋ stress. Thus it is deduced that the three genes SRTG152- I, SRTG152- Ⅱ and SRTG152- Ⅲ are related to K^＋ salt tolerance.

关 键 词：盐生植物海马齿海马 K+离子胁迫有关基因 微生物表达 功能鉴定 

分 类 号：Q943.2[生物学—植物学]