基因芯片技术在植物基因克隆中的应用研究进展  

Research Progress of Gene Chip Technology in Plant Gene Cloning

作　　者：孙兵[1,2] 闫彩霞[2] 张廷婷[2] 郑奕雄[3] 毕玉平[4] 陈高[1,2] 单世华[2] 

机构地区：[1]东北农业大学,哈尔滨150030 [2]山东省花生研究所,青岛266100 [3]山东农科院高新技术研究中心,济南250100 [4]仲恺农业工程学院,广州510225

出　　处：《基因组学与应用生物学》2009年第1期153-158,共6页Genomics and Applied Biology

基　　金：国家自然基金项目(30771361);山东省农科院创新基金项目(2006YCX012)资助

摘　　要：基因芯片是以预先设计的方式将大量的生物讯息密码(寡核苷酸、cDNA、基因组DNA等)固定在玻片、硅片等固相载体上组成的密集分子阵列。基因芯片技术本质是生物信号的平行分析,它利用核酸分子杂交原理,通过荧光标记技术检测杂交亲和与否,再经过计算机分析处理可迅速获得所需信息。由于其具有高通量、微型化、连续化、自动化、快速和准确等特点,已引起国际国内广泛的关注和重视,在许多领域得到了广泛的应用。本文简述了基因芯片的概念,技术特点及主要分类,着重对其在基因表达水平检测,基因突变和多态性的分析,基因组DNA分析,后基因组学研究以及转基因农作物检测等方面进行阐述,并说明其存在的问题及展望。Gene chips are-intensive molecular array that lots of biological message password take root with glass, silicon, such as solid-phase cartier by pre-designed manner. The parallel analysis of biological signals is the nature of gene chip technology, it detects affinity or not of hybridize by fluorescent labeling with principle of nucleic acid hybridization, it can obtain the necessary information quickly by computer analysis. Due to the characteristic of high-throughput, miniaturization, continuous, automated, rapid and accurate, become arouse a wide range of attention international and domestic, widely used in many areas. We outlines the concept, technical feature and the main classification of gene chips in this paper, emphasize detection of gene expression, gene mutation and poly- morphism analysis of the genome DNA analysis, post-genomics research and detection of genetically modified crops, and explain its problems and prospects.

关 键 词：基因芯片 基因克隆 植物 

分 类 号：Q943.2[生物学—植物学]