人参总皂苷对K562细胞促红细胞生成素受体的作用  

Effect of Total Saponins of Panax ginseng(TSPG) on Expression of Erythropoietin Receptor on Human Erythroleukemia Cell Line(K562)

作　　者：胡晓舒[1] 黄永[1] 罗春燕[1] 姜蓉[1] 王建伟[1] 

机构地区：[1]重庆医科大学组胚教研室干细胞与组织工程研究室,重庆400016

出　　处：《生物技术》2009年第1期19-21,共3页Biotechnology

基　　金：国家自然科学基金项目资助(No.30472253);重庆市教委资助课题(KJ050303)

摘　　要：目的:研究人参总皂苷(TSPG)对人红白血病细胞株(K562)促红细胞生成素受体(EpoR)的作用。方法:以50、100、200、3005、00mg/L TSPG刺激K562细胞24h,采用流式细胞仪检测细胞膜EpoR表达的变化;Elisa检测K562细胞膜、细胞浆EpoR表达量的改变;激光共聚焦显微镜观察K562细胞EpoR表达的分布。结果:以不同剂量TSPG作用K562细胞24h,流式细胞仪检测显示K562细胞膜表面EpoR呈剂量依赖性下降;细胞Elisa实验结果也显示K562细胞膜表面EpoR表达呈剂量依赖性下降,而细胞浆内EpoR表达呈剂量依赖性增加;激光共聚焦显微镜观察可见K562细胞经200mg/L TSPG作用24h后其膜上的EpoR数量明显减少,荧光强度明显减弱。结论:TSPG可使K562细胞浆EpoR的表达增强,且随着剂量的加大更加明显,而使细胞膜中EpoR的表达减少,这可能是TSPG抑制K562细胞增殖的作用机制之一。Objective：To investigate the effect of total saponins of panax ginseng（TSPG） on expression of EpoR in human erythroleukemia coil line （K562）. Method： After K562 cell induced by 50,100,200,300,500mg/L TSPG for 24 hours, respectively, EpoR expression on K562 ceil membrane was detected by flow cytometry（FCM） ,the quantity of EpoR expression in K562 cell membrane and cytoplasm was examined by Elisa, and the change of EpoR expression on K562 was observed under laser scanning confcoal microscope（LSCM）. Result：As determined by FCM analysis, the EpoR expression on K562 cell membrane decreased in a concentration - dependent manner; the results of Elisa indicated that EpoR expression decreased on K562 cell membrane, however, increased in the cytoplasma; Under LSCM, the intensity of fluorescence on K562 cell membrane became weaker obvionsly after induced by 200mg/L TSPG for 24h. Conclusion： The results suggested that TSPG induce EpoR expression on K562 cell membrane to reduce but enhance that in the cytoplasma, which may be one of mechanisms by which TSPG inhibit K562 cells to proliferate.

关 键 词：人参总皂苷(TSPG) 促红细胞生成素受体(EpoR) K562细胞 

分 类 号：R392[医药卫生—免疫学] R285.5[医药卫生—基础医学]