丙氨酸胁迫对拟南芥和烟草离体培养的影响  

Influence of Alanine Stress on Culture in Vitro of Arabidopsis thaliana and Nicotiana tabacum Linn.

作　　者：杨靖[1] 孙海燕[1] 赵元增[1] 汪英[1] 李友勇[1] 

机构地区：[1]河南科技学院生物技术系,河南新乡453003

出　　处：《生物技术》2009年第1期81-84,共4页Biotechnology

基　　金：河南省自然科学基金项目(0511031100);河南省教育厅自然科学基础研究项目(200510467019)资助

摘　　要：目的:筛选拟南芥和烟草种子离体培养的最佳培养基,并在最佳培养基下研究丙氨酸胁迫对其生长的影响。方法:在MS和1/2MS基本培养基中添加不同激素,筛选拟南芥和烟草种子的最适培养基配方,在最适培养基上加入不同浓度的D-和L-丙氨酸。结果与讨论:1/2MS不附加任何激素的基本培养基植株生长最好;D-丙氨酸对拟南芥生长有强烈抑制作用,L-丙氨酸则对生长无显著影响;D-型和L-型丙氨酸均对烟草有强烈抑制作用,这与经典的反馈抑制机理不很一致。Objective： To screen the formula of Arabidopsis thaliana and Nicotiana tabacum Linn. tissue culture for the seeking of the best media in vitro,and to study influence of alanine stress on them using the best media.Method： Adopt the MS and 1/2 MS basic culture medium with different hormone to screen the optimized culture medium formula, and added D - Ala and L- Ala of different concentration to best medium. Result ＆ Condusion： 1/2MS Basic Culture Medium without hormone was the most suitable medium for the growth of plants. Furthermore, growth of Arabidopsis thaliana was inhibited by exogenous D- Ala but not L- Ala; growth of Nicotiana tabacum Linn. wasn＇t inhibited by exogenous D- Ala or L - Ala, but the phenomenon wasn＇ t accord to classical theory of Feedback - inhibition.

关 键 词：拟南芥 烟草 培养基 丙氨酸 反馈抑制 

分 类 号：Q943.1[生物学—植物学]