红豆杉提取紫杉醇后残渣中紫杉烷类成分的含量测定  

Determination of Taxanes in the Residues Extracted Paclitaxel from Taxus

作　　者：阚常榕[1] 崔炯谟[1] 王继文 王文泽 赵余庆[3] 

机构地区：[1]延边大学,吉林延吉133000 [2]沈阳天峰生物工程技术有限公司,辽宁沈阳110016 [3]沈阳药科大学,辽宁沈阳110016

出　　处：《中国现代中药》2009年第2期27-28,44,共3页Modern Chinese Medicine

基　　金：稀有抗肿瘤活性成分的工业化制备(2007402055)

摘　　要：目的:测定红豆杉氯仿提取紫杉醇后残渣中紫杉烷类成分的含量。方法:用紫外分光光度法测定氯仿提取紫杉醇后红豆杉残渣的醋酸乙酯、正丁醇提取物的紫杉烷类成分的含量,测定波长为227nm。结果:3种红豆杉残渣的醋酸乙酯提取液中紫杉烷类成分的含量测定结果为东北红豆杉>南方红豆杉>曼地亚红豆杉,东北红豆杉树皮>东北红豆杉枝叶;正丁醇提取液中紫杉烷类成分的含量测定结果为曼地亚红豆杉>南方红豆杉>东北红豆杉,东北红豆杉树皮>东北红豆杉枝叶。结论:常法提取紫杉醇后的红豆杉残渣中仍留有紫杉烷类成分,可作为二次开发利用的再生资源。Objective： To determine the contents of taxanes in the residues extracted paclitaxel from taxus. Methods： The contents of the taxans in the residues extracted paclitaxel with CHCl3 from three species of taxus were determined by UV spectrophotometry with a detection wavelength at 227nm. Results： The contents of taxanes extracted with acetoacetate from three species of stems and leaves of the taxus were Taxus cuspidata 〉 Taxus chinensis Var. mairei 〉 Taxus Media cv. Hicksii, the barks of the Taxus cuspidata 〉 the stems and leaves of the Taxus cuspidata; the contents of taxane extracted with n-butanol were Taxus Media cv. Hicksii 〉 Taxus chinensis Var. mairei 〉 Taxus cuspidata, the barks of the Taxus cuspidata 〉 the stems and leaves of the Taxus cuspidata. Conclusion： Abound to taxanes in the residue extracted paclitaxel from taxus , it can be taken as a reused resource for further development.

关 键 词：红豆杉残渣 紫杉烷 紫外分光光度法 

分 类 号：R979.1[医药卫生—药品] R971.2[医药卫生—药学]