改良单人操作“双袖套法”建立大鼠原位肝移植模型  

Establishment of rat orthotopic liver transplantation model with modified two-cuff technique by a single operator

作　　者：黄德荣[1] 吴忠均[1] 朱宇[1] 

机构地区：[1]重庆医科大学附属第一医院肝胆外科,重庆400016

出　　处：《国际外科学杂志》2009年第2期86-89,F0003,共5页International Journal of Surgery

摘　　要：目的探讨单人操作高效、稳定地建立大鼠原位肝移植模型的技术改进。方法参照Kamada“二袖套”法，主要对供肝的灌注、切取、修整和受体缝合等进行改良，分别用传统方法（简称对照组）与改良后的方法（简称为实验组）行大鼠原位肝移植80例，比较两组间成功率及1周、1月的存活率。结果实验组与对照组比较，实验组在供体手术时间、受体手术时间、无肝期时间均较对照组明显缩短（P〈0．05），其在1周存活率、1月存活率均较对照组明显提高（P〈0．05）。结论该方法能有效的缩短供、受体手术时间，提高了供肝质量，明显提高了受体存活率，是一种稳定、可靠、有效的建立大鼠肝移植动物模型的方法。Objective To explore the modified of methods and techniques to establish a rat model of orthotopic liver transplantion effectively by a single operator,and improve the stability. Methods On the basis of two-cuff technique of Kamada,we improved the techniques of perfusing, shearing, triming donor liver, the suturation of the superior and inferior caval veins, and so on. Operation was performed in 80 rats with modified techniques （experiment group） and conventional techniques （control group）, respectively, compare the survival rates of 48 h, lweek, lmonth were compared between the two groups. Results In contrast to conventional techniques, the modified techniques reduced the average time of donor operation, recipient operation and anhepatic phase （ P 〈 0. 05 ） , elevated 1-week, 1-month survival rates, remarkably （ P 〈 0. 05 ）. Between the two groups, the survival rate of 48 hour had no statistical significance （P 〉 0. 05 ）. Conclusions The modified techniques may reduce the operation time of donor and receptor effectively raise the quality of donor liver, elevated the survival rate of receptor. It is a stable, reliable and effective method to establish the rat orthotopic liver transplantation model.

关 键 词：肝移植 大鼠 动物模型 

分 类 号：R657.3[医药卫生—外科学] R574.62[医药卫生—临床医学]