检出五种革兰氏阴性杆菌对常用的抗生素耐药情况的分析  

作　　者：崔洁[1] 宋玉文[1] 迟景宏[1] 王娟[1] 

机构地区：[1]齐齐哈尔医学院附属第三医院药剂科,黑龙江齐齐哈尔市161000

出　　处：《中国医疗前沿》2009年第1期91-91,共1页China Healthcare Innovation

摘　　要：为观察自2005年初至2006年8月期间各临床科室送检的标本,其中包括痰、尿液、腹水、胆道引流脓汁及血液标本,分别进行培养及药敏检测。方法由我院微生物室提供,以2005年初至2006年8月份药敏实验结果为样本,使用细菌鉴定药敏分析仪,符合(Nccls)标准药敏组合,可报告最低抑菌浓度(MIC)法,三个梯度(敏感、中介、耐药)的药敏实验统计数据为依据。结果共分离出473株革兰氏阴性菌。其中铜绿假单胞菌占6.76%、阴沟肠杆菌占7.39%、大肠埃希菌占18.0%、聚团肠杆菌占30.7%、奇异变形杆菌占13.7%、肺炎克雷伯菌占13.7%(臭鼻克雷伯菌占4.43%、产酸克雷伯菌占10.14%)。结论通过本实验表明,多数细菌耐药率升高,提示临床应重视抗生素的合理使用,避免滥用,遏止细菌耐药,减少不良反应发生。Objective To investigate the drug sensitivity of bacteria culture in specimens of sputum, urine, ascites, pus of biliary drainage and blood from everyclinical partment during the the years of early 2005 and the august of 2006. Methods Three gradient method of minimum inhibitory concentration was used for the antimicrobial susceptibihy test and the results were read based on national committee for clinical laboratory standards （ncels）. Results 473 strains of gram negative bacteria were isolated among which the pscudomonas aeruginosa was 6.76%, enterobacter cloacae 7.39%, cscherichia coli 18.0%, E.agglomerans was 30.7%, proteus mirabilis 13.7%, klebsiella pneumonia 13.7%（klebsiella penumoniae subsp. ozaenae4.43%, klebsiella oxytocal0.14%）. Conclusion The present study shows the rate of bacterial drug resistance increased suggesting that correct application of antibiotics should be emphasized to control and prevent resistant bacteria.

关 键 词：抗生素 革兰氏阴性杆菌 药敏最低抑菌浓度 

分 类 号：R978.1[医药卫生—药品]