大鼠脑外伤诱导反应性星形胶质细胞表达水通道蛋白1和水通道蛋白9  

Aquaporin-1 and Aquaporin-9 Expressed by Reactive Astrocytes after Traumatic Brain Injury in Rat

作　　者：张琳[1] 袁芳[2] 

机构地区：[1]首都医科大学附属北京天坛医院科研处,北京市100050 [2]北京市神经外科研究所病理生理室,北京市100050

出　　处：《中国康复理论与实践》2009年第2期129-131,I0002,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京市自然科学基金资助项目(No.7062012);国家自然科学基金资助项目(No.30840083)

摘　　要：目的研究水通道蛋白1(AQP1)和水通道蛋白9(AQP9)在大鼠自由落体脑损伤后的表达变化以及与脑水肿的关系。方法复制大鼠自由落体脑损伤模型,在外伤后1h、6h、24h、48h和168h用干重-湿重法测定损伤侧脑组织水含量,利用免疫组化染色和Western印迹法检测AQP1和AQP9表达的变化。结果脑外伤后损伤侧脑组织水含量增加,24h达高峰,168h接近恢复。脑外伤后6h损伤周围出现AQP1和AQP9免疫染色阳性的星形胶质细胞,外伤后24h免疫染色阳性细胞数最多,且损伤周围处血管内皮细胞AQP1表达异常增加。结论脑外伤诱导AQP1和AQP9的表达,共同参与脑水肿的发展过程。Objective To investigate the expression changes of aquaporin -1 （AQP1） and aquaporin-9 （AQP9） in rats after trau matic brain injury, and explore the roles of AQP1 and AQP9 in the development of brain edema. Methods In an impact- acceleration heacl injury model of rat, brain water content was measured by wet-dry weight method at 1 h, 6 b, 24 h, 48 b and 168 h after brain trauma. The expressions of AQP1 and AQP9 in brains were investigated by immunohistochemistry and Western blot. Results Brain water content increased in the injuried brain tissues after 1h post-injury, reached peak at 24 h and returned at 168 h. Both expressions of AQP1 and AQP9 were induced in reactive astrocytes adjacent to injury sit at 6 h and 24 h after brain trauma. Staining of AQP1 in the endothelial cells was not present in normal rat brains but appeared strong after brain trauma. Conclusion A remarkable induction expression of AQP1 or AQP9 after brain trauma may participate in the development of brain edema.

关 键 词：脑损伤 脑水肿 水通道蛋白1 水通道蛋白9 

分 类 号：R651.1[医药卫生—外科学]