微过氧化物酶-11在多壁碳纳米管修饰玻碳电极上的电化学  

Electrochemistry of microperoxidase-11 on a glass carbon electrode modified with multi wall carbon nanotubes

作　　者：王琨琦[1,2] 马中苏[2] 

机构地区：[1]长春工程学院理学院,长春130012 [2]吉林大学生物与农业工程学院,长春130025

出　　处：《长春工程学院学报（自然科学版）》2008年第4期80-83,共4页Journal of Changchun Institute of Technology：Natural Sciences Edition

基　　金：国家自然科学基金资助(20433060)

摘　　要：利用物理吸附的方法将微过氧化物酶-11(MP-11)固定在多壁碳纳米管(MCNT)修饰的玻碳电极表面。研究发现:在pH=7.12磷酸盐缓冲溶液中,修饰电极上的MP-11发生了两电子一质子准可逆的氧化还原反应,式量电位E0’为-298mV(vs Ag/AgCl),峰电位差ΔEP为39mV。在该修饰电极上MP-11对氧气(O2)和过氧化氢(H2O2)都能进行催化还原,催化还原过程都是扩散控制的过程。而且在信噪比为3时,MP-11对H2O2的最低检出限是0.35mmol/L,表明MP-11在修饰电极上保持了自身的生物活性,该修饰电极有望在生物燃料电池和生物传感器中得到应用。Microperoxidase - 11 （ MP - 11 ） is immobilized on a glassy carbon electrode （GCE） modified with multiwall carbon nanotubes by physical adsorption method. It was found that MP - 11 on the modified electrode takes place a quasi - reversible two electron redox reaction with a formal potential （E0＇） of - 298mV and AEP is 39mV in phosphate buffer solution （PBS） （pHT. 12）. The MP- 11on the modified electrode can catalyze the reduction O2 and H2O2. The catalytic process of O2 and H2O2 are diffusion - controlled process. The detection limit of H2O2 is 0.35mmol/L at the signal to noise ratio of three. It is obvious that bioactivity of MP - lion the modified electrode with MWCNTs is kept. The modified electrode can be used in the biofuell cell and biosensor in the future.

关 键 词：微过氧化酶-11 多壁碳纳米管 电催化 

分 类 号：O614[理学—无机化学]