卡介苗ERP基因缺失突变株免疫原性的研究  

Studies on Immunogenicity of ERP Gene Mutated BCG

作　　者：李蕾[1] 张万江[1] 吴芳[1] 王萍[1] 杜燕[1] 

机构地区：[1]石河子大学医学院,新疆石河子832002

出　　处：《石河子大学学报（自然科学版）》2008年第5期529-531,共3页Journal of Shihezi University(Natural Science)

基　　金：新疆兵团博士资金项目(04JC09)

摘　　要：为了探究卡介苗ERP基因缺失突变株对小鼠免疫应答的影响,明确敲除ERP基因后是否影响卡介苗的免疫原性,采用卡介苗ERP基因缺失突变株、卡介苗(BCG)及生理盐水背部皮下免疫小鼠,4周、8周、12周、16周后,分别采用CCK8检测脾脏淋巴细胞增殖转化率,ELISA检测淋巴细胞培养上清液IL-2、IFN-γ的产量。结果表明:卡介苗ERP基因缺失突变株组脾淋巴细胞的增殖能力及培养上清液中IL-2I、FN-γ的分泌量均明显高于生理盐水对照组(P<0.01),但与卡介苗组相比无明显差别(P>0.05)。卡介苗ERP基因缺失突变株可引发一定强度的特异性细胞免疫,而此效果与卡介苗相当。To investigate the immune response of ERP Gene Mutated BCG, and understand effection of BCG immunogenicity by delet ERP gene. At the various interval （4 weeks, 8 weeks, 12 weeks, 16weeks） after the mice were immunized by ERP Gene BCG, BCG and NS, the spleen lymphocyte stimulating index（SI） were tested by cell counting kit-8. IL-2, IFN-γlevels of culture supematant of spleen lymphocyte were tested by ELISA. In ERP Gene Mutated BCG group the SI and IL-2, IFN-γlevels of culture supernatant of spleen lymphocyte were significantly higher than those in the NS control group（ P 〈 0.01 ）, but had no significant difference compared with BCG group（ P 〉 0.05）. ERP Gene Mutated BCG could induce a certain degree of specific cellular immunity, but has no significant difference compared with BCG.

关 键 词：卡介苗 ERP基因 免疫原性 小鼠淋巴细胞 

分 类 号：R512.62[医药卫生—内科学]