新疆白蒜洋葱黄矮病毒的分子鉴定  

Identification on Molecules of Onion Yellow Dwarf Virus in Xinjiang

作　　者：陈栋[1] 向本春[1] 刘升学[1] 黄素芳[1] 

机构地区：[1]石河子大学农学院,新疆石河子832003

出　　处：《石河子大学学报（自然科学版）》2008年第5期572-574,共3页Journal of Shihezi University(Natural Science)

摘　　要：从新疆吉木萨尔县采集具有条纹花叶、畸形等症状的大蒜叶片提取总RNA,利用洋葱黄矮病毒的特异性引物进行RT-PCR扩增,可得到大小为1402bp片段。将获得的片段克隆到PMD18-T载体进行序列测定和同源性比较,结果表明,OYDV新疆吉木萨尔分离物为1402个核苷酸,可编码467个氨基酸,与GeneBank中的12个OYDV核苷酸序列相比,序列同源性为80%～99%,由此推导的氨基酸序列同源性为88%～99%。Onion yellow dwarf virus（OYDV）was detected in infected garlic plant collected from Jimusaer,Xinjiang by RT-PCR.A pair of primers were designed based on the reported OYDV gene,and a fragment of about 1402bp was amplified by RT-PCR using the total RNA of the plant infected by OYDV.The products of PCR were cloned into PMD18-T and transformed into TOP10.The combined clones were sequenced and identified with the sequences of the other 12 isolates of OYDV.The result showed that the highest homology could reach 99%of nucleic acid and 99%of amino acid,which proved that the virus was OYDV.

关 键 词：洋葱黄矮病毒 大蒜 新疆吉木萨尔 RT-PCR 

分 类 号：S436.33[农业科学—农业昆虫与害虫防治]