HPLC法同时测定头皮搽剂中氯霉素和盐酸达克罗宁的含量  

Determination of Two Contents in Scalp Liniments by HPLC

作　　者：来小丹[1] 刘松青[1] 林彩[1] 贺红军[1] 胡斌[1] 

机构地区：[1]第三军医大学西南医院药学部,重庆400038

出　　处：《解放军药学学报》2009年第1期71-73,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立同时测定头皮搽剂中氯霉素和盐酸达克罗宁含量的方法。方法采用高效液相色谱法。色谱柱:Diamon-silTM(钻石)-C18(200mm×4.6mm,5μm);流动相:甲醇-水-三乙胺(58∶42∶0.1)用磷酸调pH至5.07;检测波长:280nm;柱温:30℃。结果氯霉素在2～14μg.mL-1范围内线性关系良好(r=0.9999),盐酸达克罗宁在2～14μg.mL-1范围内线性关系良好(r=0.9998);氯霉素和盐酸达克罗宁的平均回收率分别为103.4%,101.5%。结论本法分离度好,灵敏、快速、简便,可同时测定该头皮搽剂中两组分的含量。Aim To establish a method for simultaneous determination of chloramphenicol and dyclonine cydrochloride in scalp liniments. Methods The contents of chloramphenicol and dyclonine cydrochloride were determined by HPLC. Diamonsil^TM （diamond） -C18 （200mm × 4.6mm,5μm） was used ; the mobile phase was methanol-watertriethylamine （58： 42：0.1 ） ;detectine wavelength was at 280nm, and column temperature was 30℃. Results linear calibration graphs were obtained for chloramphenicol and dyclonine cydrochloride within an concentration ranges of 2 -14μg·mL^-1 （ r =0. 999 9） and 2 - 14μg·mL^-1 （ r=0. 999 8）, respectively. The average recovery was 103.32% and 101.52%, respectively. Conclusion The method is high in resolution, simple, rapid, and precise for simultaneous determination of two contents in scalp liniments.

关 键 词：氯霉素 盐酸达克罗宁 HPLC 

分 类 号：R927.2[医药卫生—药学]