春石斛杂交育种及亲缘关系的AFLP分析  

Hybridization breeding and AFLP analysis of relative relationship of Dendrobium nobile

作　　者：郑勇平[1,2] 郑泉[3] 俞继英 张瑛 范文锋 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037 [2]浙江森禾种业股份有限公司,浙江杭州310012 [3]南京农业大学生命科学学院,江苏南京210095

出　　处：《浙江林学院学报》2009年第1期137-141,共5页Journal of Zhejiang Forestry College

基　　金：浙江省科学技术重点项目(2005c22082)

摘　　要：春石斛Dendrobium nobile为亚热带花卉。为了解决种苗依赖进口的问题,从2002年开始,从国内外引进春石斛资源,从中精选符合中国国情的花色品种和单株作为杂交育种材料,从2002-2006年授粉组合共计345个,成功育苗144个,成功率41.74%,所得杂交后代5029号和5041号2个最符合育种目标。对30个引进的品种和杂交获得的品种进行荧光AFLP(扩增片断长度多态性)分析,构建春石斛新品种的DNA指纹图谱,为品种鉴定、评价和品种创新、新品种保护提供可靠方便的技术手段。Dendrobium nobile （Orchidaceae）, subtropical flower, usually blossoms in spring. It is a very important and popular potted-flower in Europe, Japan and USA. To avoid over-reliance on the seedling import, authors had introduced about 141 species, varieties, and culitvars, and selected those who were suitable for planting in China for hybrid breeding. Between 2002 and 2006, there were 345 pollination hybrid crosses, 144 of them were bred successfully and the success rate was 41.74%. In 2004, the success rate of hybrid crosses were 58.62%. 12 hybrid crosses had very excellent prospect for development. In 12 hybrid, crosses, No. 5029 and No. 5041 as two most important ones, had been put into mass production. Authors had set up the DNA fingerprinting of new varieties of Nobile-Type Dendrobium with Fluorescent AFLP （amplified fragment length polymorphism） technology, and provided a reliable and convenient technical means for identification, evaluation, protection and innovation of varieties. [ Ch, 2 tab. 3 fig. 8 ref. ]

关 键 词：园艺学 春石斛 杂交育种 AFLP分析 

分 类 号：S682.31[农业科学—观赏园艺] S603.3[农业科学—园艺学]